Objectives: 1. To fractionate the liver tissue and understand the idea of fractionation. 2.

To identify the presence of different biochemical components of the organelles. 3. To determine the composition of the body and the major classes of molecules. Analysis: Molisch Test shows that all types of carbohydrates give a positive result which leads to a purple color. In the experiment, the purple ring appears to all sediments and supernate but with varying degree. It is in the Sediment 3 and Supernate 3 that yielded the most obvious and clear purple ring. While the Sediment 2 yielded a fain purple ring color. Carbohydrates are present. Benedicts Test shows that all monosaccharide and disaccharides gives a positive result except sucrose. These monosaccharide and disaccharides reduce weak oxidizing agents that changes color from blue to reddish brown. In our experiment, almost all of the sediments solution turned to light aqua but the control solution is reddish brown; therefore, all of the tests for the sediments yielded a negative result. Biuret Test states that a violet color will appear in the solution if the amide bonds common to proteins is present and this reaction carries out a high pH. Therefore, since all of the sediments and supernate yielded a different shade of color from the control, we can say that protein is not present in any of our sediments and supernate. Xanthoproteic Test is used to identify the presence of an activated benzene ring. So a positive result will change the solutions color to yellow. In our experiment, Sediments 1 & 3 and Supernate yielded a positive result. While the Sediment 2 had a lighter color so it yielded to a negative result. So, tyrosine and tryptophan is present in Sediment 1 & 3 and the Supernate while absent in Sediment 2. Sudan series of dyes do not react chemically with lipids while the dye molecules have a hydrophobic effect. All of our sediments and supernate yielded a positive result. Since its color is almost the same with the control solution. They are all red in color. So we can say that lipids are present in a chicken liver. Acrolein Test is utilized to determine the presence of glycerin in a fat and can easily be detected by its odor. In our experiments, sediment 1 & 2 yielded a positive result because it gave off a fain odor that is similar to that of the controls while Sediment 3 and Supernate gave a negative result. We can say that glycerin is present but in few amounts in Sediment 1 & 2 while absent in Sediment 3 and Supernate.

CARBOHYDRATES Molisch Test Benedicts Test PROTEINS Biuret Test Xanthoproteic Test LIPIDS Sudan Test Acrolein Test

CONTROL 1% Ribose Purple Ring Brick Red 1% Albumin Purple Intense Yellow 1% Lecithin Red Biting Odor

SEDIMENT 1 +++

SEDIMENT 2 ++

SEDIMENT 3 ++++

SUPERNATE 3 ++++

+++ ++++ ++ ++++ +

+ ++++

+++ +++++