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Introduction to Nanobiotechnology Nano-Biosensing Ion-channel Based Sensing N Nano-optics for bi ti f biosensing i DNA as a tool in nanotechnology Conductivity of DNA Nanowire Biosensing Principles of localized surface plasmon resonance sensing (Takumi Sannomiya)
Nanobiotechnology
What is Nanobiotechnology?
Nanobiotechnology is the application of nanotechnology to life sciences. It includes: th application of nanoscale tools to biological the li ti f l t l t bi l i l systems the use of biological templates to create p nanoscale products
Nature Biotechnology 21:1137-1143 (2003)
Why Nanobiotechnology? Mother Nature did it first: A cell is a warehouse of nanoscale machines that function in a highly controlled manner. Biology can teach the physical world of electronics, materials science computing and manufacturing science, manufacturing. There exists biological analogues of conventional functional devices. We have the tools now to do it.
Nanobiotechnology Applications
Nanomedicine Nanobiosensors (biochips) Nanofluidics N fl idi Molecular Self-Assembly Intelligent Drug Delivery Systems Nanomachines
Hype or Hope?
Biosensing Concept
Enzymatic Biosensors - Principles and Applications. D. Grieshaber, R. MacKenzie, J. Vrs, and E. Reimhult, Sensors, 8(3); 1400-1458, 2008.
Current stops
Ferritin
IgG
Streptavidin
Gramicidin
No N current is detected ti d t t d
Enhancing Sensitivity
http://www.ambri.com.au
Applications
Point of care Point-of-care diagnostics Drug discovery Bacteriological detection B t i l i l d t ti Veterinary diagnostics Food testing g Environmental monitoring
Au A
Ag A
100nm
100nm
Color of Nanoparticles
Gold Nanoparticles Quantum Dots
4nm
50nm
Old-New Discovery
Surface Ag Nanostructures
Immunosensing Performance
Advantages
Simple absorption measurement Compatibility with array format Label-free technique
Applications
High through-put screening Interaction analysis
Problems
Sensitivity Theory and interpretation
DNA in Nanotechnology
Folding DNA to create nanoscale shapes and patterns Paul W. K. Rothemund, Nature 440, 297-302 (16 March 2006)
Folding DNA to create nanoscale shapes and patterns Paul W. K. Rothemund, Nature 440, 297-302 (16 March 2006)
DNA Nanotemplating
DNA-Templated Self-Assembly of Metallic Nanocomponent Arrays on a Surface NANO LETTERS, 2004, 4(12): 2343-2347
Summary
Nanobiosensors: Ion-Channel Based Sensing
100nm
100nm
Conductivity of DNA
Contact Examples
Using top-down techniques Or Carbon Nanotubes
Semiconducting M-DNA