Test-2 Based on Unit-4 of CSIR

ribosylation, pertussis inhibits the same

b)

1.
between a) b) c) d)

Hemidesmosomes are structures found two adjacent plant cells two adjacent animal cells cell and extracellular matrix within a bacteria

Cholera toxin activates Gs, pertussis toxin activates Gi c) Both work by same mechanism d) Cholera toxin activates ADP ribosylation but pertussis toxin just opens the appropriate channel 6. e) f) g) h) The P53 gene is especially prone to point mutation chromosomal rearrangement loss none of the above Nitroglycerin tablets and other nitro Vasoregulators are taken to relieve angina. Which of the statements is NOT true a) It relieves the pain caused by contraction of a heart deprived of O2 because of blocked coronary arteries b) NO-induced relaxation of cardiac muscle c) Glycerin induced relaxation of cardiac muscle d) Increased cGMP synthesis diminish after the stimulus ceases Mutations in the R or C subunit of cAMPdependent protein kinase (PKA) might to

2. Some receptors for growth factors activate a protein kinase cascade, with the participation of multiple enzymes to effect a change in gene expression. Which of the following statements about a protein kinase cascade are true?

a)

Multiple steps allow the amplification of the signal.

7.

b) c)

External signals can lead to changes in gene expression. Multiple steps leading to kinase activations can result in cells having different responses, depending on the presence or absence of target proteins.

d)

Multiple steps in an activation mean that abnormal stimulation of a cell response such as growth can occur with mutations in more than one gene.

e) 3.

All of the above are true. Destruction box is a) Sequence for protein targeting b) Sequence in cyclins amino terminal c) Region of DNA required for its destruction d) Carboxy terminal sequence of suicidal enzymes. lead

8.

a)

mutation in the R subunit makes it unable to bind to the C subunit, the C subunit is never inhibited; it is constantly active.

b)

4. The specificity of the steroid-receptor interaction is exploited in the use of the drug tamoxifen. In some types of breast cancer, division of the cancerous cells depends on the continued presence of the hormone estrogen. Tamoxifen competes with estrogen for binding to the estrogen receptor, but the tamoxifen-receptor complex has little or no effect on gene expression; tamoxifen is an antagonistof estrogen. tamoxifen administered slows or stops the growth of remaining cancerous cells b. increases the growth of cancerous cells c. unregulated growth d. no effect on the cancerous cells but effects normal cells 5. Difference in the mechanism of cholera toxin and pertussis toxin is a) Cholera toxin activates ADP

mutation prevents the binding of cAMP to the R sub unit but still allows normal R-C interaction, the inhibition of C by R cannot be relieved by elevated cAMP so constantly inactive. c) Both a and b d) None

9.
including

A variety of unusual invertebrates, giant clams, mussels, and polychaete worms, live on the fringes of deep-sea hydrothermal vents, where the temperature is 60 degrees C. The

a.

adductor muscle of a giant clam has a resting membrane potential of 95 mV. Given the intracellular and extracellular ionic compositions shown below predict membrane potential?

a. b. c. d.

-90mV 90mV -190mV 190mV

cells with damaged DNA and DNA damage checkpoint. Which of the following is NOT true a. Cells with functional p53 arrest in G1 and G2 b. Cells lacking functional p53 do not arrest in G1 c. P53 encodes a phosphorylated protein d. P53 is highly essential for normal cell division 14. Viral encoded Ras oncogene transforms normal mammalian cells into cancer cells. Viral Ras protein differs from its normal counterpart by a. Diminished GTPase activity b. Increased GTPase activity c. Diminished ATPase activity d. Increased ATPase activity 15. The most important difference between gap junctions between animal cells and plasmodesmata in plants is that in plasmodesmata a. Ionic coupling occurs b. Two adjacent plasma membranes are fused c. Metabolic cooperation occurs d. Pore diameter is 1mm 16. Intracellular receptors were identified and have been proposed to mediate actions of steroid hormones. In an experiment to study various actions of estrogen, it was covalently coupled to albumin, a protein of 68kD. When this protein coupled hormone was added to estrogen responsive cells, a measurable response was observed. This indicates that a. estrogen receptors are not found intracellularly b. estrogen coupled protein is taken up by the cells by passive diffusion c. estrogen receptors are also present on the cell membrane d. steroid hormone coupled protein is cleaved by the cells and free estrogen is taken up by the cells by passive diffusion 17. Some oncogenic retroviruses can transform normal mammalian cells to cancer cells in vitro. This transformation of a best cell is generally associated with a. Conversion of the host DNA into viral RNA b. Presence of viral particles in the hosts cell membrane c. Integration of the viral genome into the hosts nuclear DNA d. Release of numerous viral particles and

10.
pathway

Two protein kinases, K1 and K2 function sequentially in regulating intracellular in response to extracellular signal. The

following observations are made: (i) Response is observed even in the absence of extracellular signal when a mutation permanently activates K1. (ii) Response is observed even in the absence of extracellular signal when K1 contains an activating mutation and K2 with inactivating mutation. (iii) No response in the cells is detected even in the presence of extracellular signal when both kinases are inactivated by mutation. Which one of the following is correct? a) K1 activates K2 b) K2 activates K1 c) K1 inhibits K2 K2 inhibits K1 The MEK kinase is unusual in that a. It is activated by a kinase b. It lies downstream of Gprotein coupled receptors c. It is dual specificity kinase, having the ability to phosphorylate both threonines and tyrosines d. It activates kinase

d)
11.

12. If a mouse is repeatedly immunized with a complex protein such a bovine serum albumin (BSA), the resulting serum antibodies will be of a. Identical isotype and identical antigen binding sites b. Various isotype but identical antigen binding sites c. Identical isotype but various antigen binding sites d. Various isotype and various antigen binding sites 13. DNA damage blocks progression through the cell cycle until the damage is repaired . Damage to DNA can result from chemical and physical agents. Tumour suppressor protein , p53 contributes to arrest of

concomitant death of host cells 18. Which of the following is/are correct about apoptosis? p. bcl-2 is an antiapoptotic gene q. cytochrome C activates intrinsic apoptotic pathway r. it is mediated by protease called caspases s. it is a reversible process mediated by serine protease a. p and q b. p and r c. q, r, s d. p,q,r

c. Membrane attack complex can get inactivated by the nucleated d. Membrane attack complex can get inactivated cells hence cannot get inserted in the nucleated cell membrane 23. Caspases a. Synthesized in the cell as inactive precursors b. Are cysteine proteases c. Cleave protein at cysteine amino acid residue d. A and b are correct

24. Retroviruses are capable of causing 19. It was found that normal stem cells cancer because they isolated from brain have a doubling time of 24 hrs, a. Produce a very high number of whereas tumor derived cells have a doubling time of 12 progeny viruses per infected cell hrs. Both these cell types were grown in normal media. At b. Often contain point mutations in the end of a week, radioactivity per cell will be their pol gene a. Double in stem cells as c. Tranduce mutant versions of compared to cancer cells cellular genes that normally b. Half in stem cells as compared regulate cell growth to cancer cells d. Infect cells more efficiently than c. Same in both stem and cancer other viruses cells d. None in stem cells and high 25. Integrins are characterized by which of levels in cancer cells the following properties? p. are heterodimeric protein 20. A point mutation in a newly identified q. bind to the tripeptide sequence Argreceptor tyrosine kinase renders it constitutively active. Gly-Asp Which of the following is NOT true? r. are peripheral membrane proteins a. It is a gain of function mutation s. are expressed in a cell specific manner b. Mutant kinase can function in a ligand a. p and q independent fashion b. q and s c. It can contribute to carcinogenesis c. p, q and s d. The mutation is recessive d. p, q, r and s 21. Which of the following is NOT a property of the mammalian signal recognition particle? a. It targets nascent secretory polypeptides to the rough endoplasmic reticulum b. It binds to the signal sequences of secretory proteins c. It contains both RNA and several proteins d. It contains a signal peptidase activity 22. Nucleated cells tend to be more resistant to complement mediated lysis than RBC because a. Many nucleated cells can endocytose the membrane attack complex b. Membrane attack complex cannot get inserted in the nucleated cells