^PALL) Life Sciences

Animal Health

Equine Platelet Enhancement Therapy - E-PET

E-PET makes platelet therapy simple

Platelet therapy can promote healing in tendon or ligament injuries and joint diseases, such as osteoarthritis. Pali's E-PET system makes platelet therapy simple. The secret is E-PET's unique filter-based approach to concentrating platelets and their associated growth factors. Preparation and treatment is easy. No centrifuge, power supply, or outside lab processing required * As easy to use in the field as it is in the clinic Single-use, disposable system is cost-effective and efficint Gravity filtration is gentle on platelets, creating a high quality platelet therapy rich in growth factors 6 ml_ sample volume provides the recommended dose for most treatment regimens

Filtration. Separation. Solution.su

Procedure
E-PET makes platelet therapy simple. Blood is drawn and loaded into a bag above the E-PET filter. Blood flows through the filter under gravity. As platelets reach the filter, they are selectively retained. When the filtration is complete, a harvest solution is backflushed through the filter to recover the platelets. This harvested product is the platelet therapy, and is ready to use. It's that simple.

Filtration and Recovery

As blood flows through the E-PET system, platelets are selectively captured on the surface of the filter through a complex interaction of size exclusion and adsorption, while the majority of red cells pass through.

The platelet concentrate is then recovered by back-flushing the filter with a harvest solution.

Recover the cell therapy

Deliver the cell therapy

T
Performance
E-PET is a system that provides a 7x concentration of platelets (PLT) and their associated growth factors (GF) from a small volume of your horse's own blood.
1250-,
-35 -30 ^
-25g

Since the horse is the donor and recipint, there are no immune reactions to the platelet concentrate.

.= 1000V)

750H

o o 500.

20 S
15 10

g | 3

250-

IL
Blood

-5

E-PET

Blood

E-PET

*Growth factor release after activation with Platelet Activating Factor (PAF).

(PALL) Life Sciences ^^ -^

Animal Health
www.pall.com/AnimalHealth Pall Corporation 25 Harbor Park Drive Port Washington, NY 11050 1.516 801 9738 customer service animalhealth@pall.com e-mail
International Offices Pall Corporation has offices and plants throughout the world in tocations such as: Argentina, Australia, Austria. Belgium, Brazil, Canada, China, France, Germany, India, Indonesia, Ireland, Itaiy, Japan, Korea, Malaysia, Mexico, the Netherlands, New Zealand, Norway, Poland, Puerto Rico, Russia, Singapore, South Africa, Spain, Sweden, Switzeriand, Taiwan, Thailand, the United Kingdom. the United States and Venezuela. Distributors in alt major industrial areas of the world. The information provided in ths literature was reviewed for accuracy at the time of pubtication. Product data may be subject to change without notice. For current information consult your local Pall distributor or contact Pali directly. 2011. Pall Corporation. Pall, and (PALL), are trademarks of Pall Corporation. indicates a trademark registered in the USA and TM indicates a common law trademark. Fiftration.Separation.Solution. is a service mark of Pall Corporation.

Filtration. Separation. Solution.

5/11, 1K GN11.6772

Life Sciences
Instructions for Use
Reorder code: E-PET

Equine Platelet Enhancement Therapy (E-PET)

This product is used to create a platelet therapy from autologous equine blood. E-PET application is as easy as 1, 2, 3

1. Prpar

2. Filter

3. Recover

Filter the blood at stall-side through the E-PET system. Recover the platelets and deliver the therapy to the site of injury or disease within 60 minutes.

E-PET - Instructions for Use

Equipment Provided
E-PET System comprising: 1 - E-PET set 1 - Anticoagulant Solution (ACD-A) 1 - Capture Solution (sterile water for injection) 1 - Harvest Solution 3 -10 m L Luer lock syringes 1 - 60 m L Luer lock syringe 1 - Clean drape Filtration 1. Once the blood sample and Capture Solution are mixed well, re-hang the E-PET Set in a vertical position with the top bag above the filter.the arrows on the filter pointing downward, and the bag hanging freely. 2. Open the bottom clamp and then the top clamp to allow the whole blood to flow through the filter into the lower bag. Filtration should take between 5 and 14 minutes. (Co/c/ environmental temperatures may cause slower flow rates.) 3. Once the filtration is complete (when the unprinted side of the filter is free of fluid and flow has stopped) push the bottom and top clamps into the closed position. Recovery of Platelets 1. Withdraw 8 ml_ Harvest Solution into one of the 10 ml_ syringes. 2. Attach this syringe to Port C. The platelets concentrate wiil be collected in the remaining empty 10 ml_ syringe. This syringe should be handled in a sterile fashion if full sterility is required for the application/implantation procedure. 3. Remove any residual air from the remaining empty 10 ml_ syringe by depressing the plunger. 4. Attach this empty syringe to Port B. 5. Holding the filter with one hand, rapidly inject the Harvest Solution through the filter with constant pressure. This will take approximately 2-4 seconds. 6. If the plunger does not depress easily, remove syringe(s) and then reattach. 7. The platelets will be back-flushed into the empty 10 mL syringe attached to Port B. 8. Remove the syringe containing the platelet concentrate from Port B. 9. The platelet therapy is now ready for use and should be applied/implanted within 60 minutes of recovery. Additional training and consultation available at Pall by calling, 516-801 -9727 or emailing Jeffrey_Schaffer@pall.com

Pt

Equipment Required
1 - Needie for aspiration of blood 3 - Needies for aspiration of anticoagulant, capture, and harvest solutions Equipment for clipping and surgical preparation of aspiration site. Equipment for implantation/application

How to Recover Concentrated Platelets

Preparation 1. Place clean drape on a flat surface. 2. Remove E-PET set from box and lay it on the drape. (If Harvest Solution is very co/d put bottle into pocket before use.) 3. NOTE: Push the top and bottom clamps into the closed position. 4. Preload the 60 ml_ syringe with 5 ml_ anticoagulant solution. 5. Withdraw 9 ml_ of capture solution into one of the 10 ml_ syringes and then transfer the capture solution into the top bag via Port A. 6. Remove the syringe and replace the cap on Port A. 7. Hang the E-PET set so that the bag containing the capture solution is above the filter, the arrows on the filter point downward, and the bags hang freely. Check that both top and bottom clamps are closed. Blood Collection 1. Collect 55 ml_ whole venous blood into the preloaded 60 ml_ syringe, giving a total volume of 60 ml_. 2. Attach the syringe to Port A and transfer the anticoagulated whole blood to the top bag while gently mixing it with the capture solution by rocking the bag. 3. Remove the syringe and replace the cap on Port A. 4. Continue mixing the blood and capture solution by removing the E-PET set from its hanging position and inverting the bag set at least 10 times. 5. Good results require that the sample and capture solution are wel l mixed.

PALL) Life Sciences

Manufactured for: Pall Corporation 25 Harbor Park Drive Port Washington, NY 11050 www.pall .com/animalhealth For customer service oall: 1.516.801.9738 by: Medsep Corporation A subsidiary of Pall Corporation Covina, CA 91722

Visit us on the Web at www.pall.com/animalhealth

International Offices Pall Corporation has offices and plants throughout the wcrld ih locations such as: Argentina, Australia, Austria, Belgium, Brazrt, Canada, China. Frarice, Germany, Indra, Indonesia. Iretand, Italy, Japan, Korea, Malaysia, Mexico, the Netherlands, New Zeaiand, Norway, Poland, Puerto Rico, Russia, Singapore, South Africa, Spain, Svveden, Switzenand. Tawan, Thailand, the United Kingdom, the United States and Venezuela. Distributors in all maior industrial areas of the worid. The information provided in this literature was reviewed lor accuracy at the time of pubiication. Product data may be subject to change without notice. For current information consult your focal Pall distributor or contact Pal: directly.

2011, Pall Corporatkxi. Pall, and (P*!$> are trademarks of Pall Corporation. indicates a trademark registered in the USA. Filtratkin.Separation.Solution.su is a service mark of Pall Corporation. 4/11, PDF, GN09.3298 PN147EPETO