Professional Documents
Culture Documents
Function
Immunoglobulins:Structure and
Function
Definition: Glycoprotein molecules that are
produced by plasma cells in response to an
immunogen and which function as antibodies
Amount of protein
albumin
globulins
Immune serum
Ag adsorbed serum
Mobility
B Cells
work chiefly by secreting
soluble substances known
as antibodies (Ab)
General Functions of
Immunoglobulins: What do Ab do?
Ag binding
Can result in protection
Valency
Immunoglobulin Structure
Heavy & Light
Chains
Disulfide bonds
Inter-chain
Intra-chain
Disulfide bond
Carbohydrate
CL
VL
CH2
CH1
VH
Hinge Region
CH3
Immunoglobulin Structure
Disulfide bond
Variable &
Constant
Regions
VL & CL
VH & CH
Hinge Region
Carbohydrate
CL
VL
CH2
CH1
VH
Hinge Region
CH3
Immunoglobulin Structure
Domains
Disulfide bond
VL & CL
VH & CH1 - CH3
(or CH4)
Oligosaccharides
Carbohydrate
CL
VL
CH2
CH1
VH
Hinge Region
CH3
IgG molecule
Variability Index
100
HVR2
HVR1
50
FR2
FR1
25
FR3
75
50
Amino acid residue
Framework regions
FR4
100
Immunoglobulin Fragments:
Structure/Function Relationships
Fab
Papain
Ag binding
Valence = 1
Specificty
determined by VH
and VL
Fc
Effector
functions
Fc
Fab
Immunoglobulin Fragments:
Structure/Function Relationships
Ag Binding
Immunoglobulin Fragments:
Structure/Function Relationships
Fab
Pepsin
Ag binding
Fc
Effector functions
F(ab)2
Fc
Peptides
F(ab)2
Human Immunoglobulin
Subclasses
IgG Subclasses
IgG1 - Gamma 1 (1) heavy chains
IgG2 - Gamma 2 (2) heavy chains
IgG3 - Gamma 3 (3) heavy chains
IgG4 - Gamma 4 (4) heavy chains
IgA subclasses
IgA1 - Alpha 1 (1) heavy chains
IgA2 - Alpha 2 (2) heavy chains
Human Immunoglobulin
Light Chain Types
Kappa ()
Lambda ()
Human Immunoglobulin
Light Chain Subtypes
Lambda light chains
Lambda 1 (1)
Lambda 2 (2)
Lambda 3 (3)
Lambda 4 (4)
Immunoglobulins
Nomenclature
IgM (kappa)
IgA1(lambda 2)
IgG
Heterogeneity
IgG
Structure
Monomer
IgG3
IgG
Structure
Properties
Major serum Ig
Major Ig in extravascular spaces
Placental transfer Does not require Ag
binding ( IgG2)
Fixes complement ( IgG4)
Binds to Fc receptors ( IgG2, IgG4)
Phagocytes - opsonization
K cells - ADCC
IgM
Structure
Pentamer (19S)
Extra domain
(CH4)
J chain
J Chain
C4
IgM
Structure
Properties
3rd highest serum Ig
First Ig made by
fetus and B cells
Fixes complement
No activation
Activation
IgM
Structure
Properties
3rd highest serum Ig
First Ig made by
fetus and B cells
Fixes complement
Agglutinating Ig
Binds to Fc
receptors
B cell surface Ig
Tail
Piece
Ig- Ig-
Ig- Ig-
IgA
Structure
Serum - monomer
Secretions (sIgA)
Dimer
J chain
Secretory
component
Secretory Piece
J Chain
IgA
Structure
Properties
2nd highest serum Ig
Major secretory Ig (Mucosal or Local Immunity)
Tears, saliva, gastric and pulmonary secretions
IgD
Structure
Monomer
Tail piece
Tail Piece
IgD
Structure
Properties
4th highest serum Ig
B cell surface Ig
Does not bind complement
IgE
Structure
Monomer
Extra domain
(CH4)
C4
IgE
Structure
Properties
Least common serum Ig
Binds to basophils and mast cells (Does not
require Ag binding)
Allergic reactions
Parasitic infections (Helminths)
Binds to Fc receptor on eosinophils
Immunoglobulin:
Isotypes, Allotypes and Idiotypes
Immunoglobulin Isotypes
Definition - Antigenic determinants that
characterize the classes and subclasses
of heavy chains and types and subtypes of
light chains
Immunoglobulin Isotypes
Definition
Location
Occurrence
Importance
Ig levels
B cell tumors
Immunodeficienci
es
IgG1 (kappa)
IgM (kappa)
Immunoglobulin Allotypes
Definition - Antigenic determinants specified
by allelic forms of the Ig genes
Location
Occurrence
IgG1 (kappa)
IgG1 (kappa)
Person 1
Person 2
Domain
IgG1
CH1
G1m(f) = (3)
Arg
214
CH1
214
CH3
G1m(a) = (1)
355-358
CL
Km(1)
Arg, Asp,
Glu, Leu
Val, Leu
CL
Km(3)
Ala, Val
153, 191
light
chain
Allotype
Amino
Acid
Chain
Position
153, 191
Domain
IgG1
CH1
G1m(f) = (3)
Arg
214
CH1
214
CH3
G1m(a) = (1)
355-358
CL
Km(1)
Arg, Asp,
Glu, Leu
Val, Leu
CL
Km(3)
Ala, Val
153, 191
Km(1)/Km(3) X Km(1)/Km(1)
Allelic exclusion
light
chain
Allotype
Amino
Acid
Chain
Position
153, 191
Immunoglobulin Allotypes
Definition
Location
Occurrence
Importance
Monitoring bone marrow grafts
Forensic medicine
Paternity testing
Immunoglobulin Idiotypes
Definition - Unique antigenic determinants
present on individual antibody molecules
or on molecules of identical specificity
Immunoglobulin Idiotypes
Definition
Location
IgG1 (kappa)
IgG1 (kappa)
Person 1
Person 1
anti-A
anti-B