Professional Documents
Culture Documents
Objectives:
State the shapes and arrangements of named
examples of bacteria
Describe the appendages and inclusions found in
various examples of bacteria
Distinguish between simple, differential and
special stains
State the differences between the cell walls of
Gram positive and Gram negative bacteria and
how these relate to staining characteristics.
Describe the bacterial endospore and explain its
role and describe its role in the survival of the
bacteria.
arrangements:
streptococci: cocci
arranged in chains
(genera
Streptococcus
eg. S. pneumoniae,
S.mutans, S.
Pyogenes)
http://www.google.com.jm/search?
hl=en&site=imghp&tbm=isch&source=hp&biw=1137&bih=570&q=micrococcus+luteus+morphology&oq=micrococcus&g
s_l=img.1.3.0l4j0i24l6.2199.15199.0.18567.13.9.1.3.3.0.333.1846.0j5j3j1.9.0...0.0...1ac.1.2.img.esuYoEPN2hU#hl=en&tbo
=d&site=imghp&tbm=isch&sa=1&q=streptococcus&oq=strepto&gs_l=img.1.0.0l10.271591.277847.0.279974.20.14.0.1.1.
4.236.1517.3j7j1.11.0...0.0...1c.1.2.img.AwyvcfhNtyg&bav=on.2,or.r_gc.r_pw.r_qf.&bvm=bv.41867550,d.eWU&fp=e285e9e
c378f76ce&biw=1137&bih=570&imgrc=b_8PFVAFXAlt5M%3A%3BK4fARlycGpKszM%3Bhttp%253A%252F
%252Fupload.wikimedia.org%252Fwikipedia%252Fcommons%252Fthumb%252F2%252F2e%252FStreptococci.jpg
%252F270px-Streptococci.jpg%3Bhttp%253A%252F%252Fen.wikipedia.org%252Fwiki%252FStreptococcus%3B270%3B166
Division in two
planes produces
squares of 4 called
tetrads
arrangement.
(genera micrococcus
http://www.google.com.jm/imgres?
q=micrococcus+luteus+morphology&hl=en&tbo=d&biw=1137&bih=570&tbm=isch&tbnid=XjkreccgzOx_jM:&imgrefurl=http://mail.gib
raltar.k12.wi.us:8100/~jdickson/AP_Biology/Lab13_Bacteria/Lab13.htm&docid=qftzYq3uGw1teM&imgurl=http://mail.gibraltar.k12.wi.us:
8100/~jdickson/AP_Biology/Lab13_Bacteria/images/micrococcus.jpg&w=320&h=182&ei=XX0OUetBIG68wSlzYGwCA&zoom=1&ved=1t:3588,r:11,s:0,i:112&iact=rc&dur=1495&sig=102320833935158558452&page=2&tbnh=144&tbn
8
(Genera Sarcina eg. S. ventriculi
and S. lutea)
http://www.google.com.jm/imgres?
q=streptobacillus&hl=en&tbo=d&biw=1137&bih=570&tbm=isch&tbnid=
2BuKBayZzPczcM:&imgrefurl=http://student.ccbcmd.edu/courses/bio141/l
abmanua/lab1/stbac.html&docid=ri4ifNWmiH3QiM&imgurl=http://faculty.c
cbcmd.edu/courses/bio141/labmanua/lab1/images/streptobacillus1_scale_
final.jpg&w=360&h=282&ei=6pIOUZ30NIbM9gTb6IDwAg&zoom=1&ved=
1t:3588,r:1,s:0,i:79&iact=rc&dur=17&sig=102320833935158558452&pa
ge=1&tbnh=181&tbnw=231&start=0&ndsp=10&tx=157&ty=46
in chains
a coccus
(eg. Haemophilus influenzae and
Chlamydia trachomatis )
http://www.google.com.jm/imgres?
q=coccobacillus&hl=en&tbo=d&biw=1137&bih=570&tbm=isch&tbnid=JsPhWutVCsmJM:&imgrefurl=http://jpkc.njau.edu.cn/spwswx/cankao/ShowArticle.asp%3FArticleID
%3D314&docid=6WXUDE7spXHX9M&imgurl=http://jpkc.njau.edu.cn/spwswx/imgbank/t
uku/Coccobacillusshaped%252520Bacterium(causes%252520respiratory
%252520infections)%252520Bordetella
%252520holmesii.jpg&w=500&h=400&ei=kJMOUfPkH4Ts9AT96IDICg&zoom=1&ved=1t:
3588,r:3,s:0,i:87&iact=rc&dur=7&sig=102320833935158558452&page=1&tbnh=185&
tbnw=231&start=0&ndsp=10&tx=93&ty=137
Spiral
a. vibrio: a curved or comma-
shaped rod
(eg. V. cholerae and V.
parahaemolyticus)
b. spirillum: a thick, rigid spiral
(eg. Spirillum minus)
c. spirochete: a thin, flexible spiral
http://www.google.com.jm/imgres?
q=sheathed+bacteria&hl=en&tbo=d&biw=1137&bih=570&tbm=isch&tbnid=mOSKAxeTZpB4M:&imgrefurl=http://classes.midlandstech.edu/carterp/courses/bio225/chap04/lecture2.htm&docid=PUFIB
AFoV-gixM&imgurl=http://classes.midlandstech.edu/carterp/courses/bio225/chap04/0404_SpiralBacteria_1.jpg&w=513&h=679&ei=cpsOUcLJDorm9ASR3YDABA&zoom=1&ved=1t:3588,r:22,s:0,i:152&i
Sphaerotilus)
Stalked (Gallionella ferruginea)
Filamentous (Herbidospora
cretacea)
square (Walsbys square
bacterium)
star-shaped
spindle-shaped
lobed, and (hyphomicrobium)
pleomorphic
http://en.wikipedia.org/wiki/Bacterial_cellular_morphologies
http://
www.google.com.jm/imgres?q=Walsby%E2%80%99s+square+bacterium&hl=en&tbo=d&biw=1137&bih=570&tbm=isch&tbnid=sbuS2iCG_IfYM:&imgrefurl=http://microbewiki.kenyon.edu/index.php/Haloquadra&docid=YUW mwhLQpyVzcM&imgurl=http://microbewiki.kenyon.edu/images/c/c4/FourSquares_
AO_vs.jpg&w=154&h=154&ei=ZZoOUeDCMone8ATFxI GYCA&zoom=1&ved=1t:3588,r:5,s:0,i:92&iact=rc&dur=17&sig=102320833935158558452&page=1&tbnh
=122&tbnw=113&start=0&ndsp=11&tx=32&ty=12
Pilli
are straight hair-like
http://www.google.com.jm/imgres?
q=bacteria+with+pili&hl=en&tbo=d&biw=1137&bih=570&tbm=isch&tbnid=0Hkq_Baj1NJQM:&imgrefurl=http://micro.magnet.fsu.edu/cells/bacteriacell.html&docid
=XaGl3hG0jC1k5M&imgurl=http://micro.magnet.fsu.edu/cells/procaryotes/images/pro
caryote.jpg&w=375&h=430&ei=opwOUbf1H4ee8QTFjYD4BA&zoom=1&ved=1t:3588
,r:1,s:0,i:81&iact=rc&dur=5&sig=102320833935158558452&page=1&tbnh=192&tb
nw=167&start=0&ndsp=11&tx=88&ty=114
Flagellum
inner
S(superficial) and M (membrane) rings.
Bacterial Flagella
Flagellum
Different types of
bacteria have
different numbers of
flagella:
Monotrichous
(genera
pseudomonas),
amphitrichous,
lophotrichous and
peritrichous
(genera
Escherichia).
Capsule
Most bacteria secrete a slimy or gummy
out
Adhere cells to a surface where conditions
Sheath
Some bacteria develop within sheaths
(sheathed bacteria)
Nucleoid
The nucleoid or nuclear region is well defined
information.
Bacterial DNA (chromosomal) is usually
Ribosomes
Found in the cytoplasm.
Their great number and small size give the
Storage granules
Many bacterial species have several kinds
of storage granules.
Other inclusions
Gas vacuoles: gas-filled regions
Magnetosomes:
These magnet-like structures are needed
for magnetotaxis.
They allow the bacteria to follow magnetic
Heterocysts:
Nitrogen fixation and oxygenic
Heterocyst
Stains
Mycobacterium.
of the stain must be near boiling and the stain cannot dry
out.
Differential Stain
The Gram Reaction
The Gram reaction is named after the Danish
Gram-negative cells is their ability to retain the crystal violetiodine complex when treated with a solvent.
Cell wall
Bacterial wall is made mostly of a rigid
Peptidoglycan is composed of N-
http://www.google.com.jm/imgres?q=gram+positive+and+gram+negative+bacterial+cell+wall+structure&um=1&hl=en&sa=N&tbo=d&biw=1137&bih=570&tbm=isch&tbnid=UtTPQoAW33fD9M:&imgrefurl=http://tommytoy.typepad.com/tommy-toy-pbtconsultin/2011/11/antibacterials-startups-draw-venture-capital-for-drugs-targeting-gram-nevagive-bacteria-and-infectio.html&docid=Xq4WMe8QAdqnVM&imgurl=http://tommytoy.typepad.com/.a/6a0133f3a4072c970b0162fc6173d0970d550wi&w=445&h=312&ei=O6oPUeO5MoT49QTQwoDQCQ&zoom=1&ved=1t:3588,r:1,s:0,i:82&iact=rc&dur=1681&sig=102320833935158558452&page=1&tbnh=182&tbnw=267&start=0&ndsp=12&tx=114&ty=61
Endospores
Endospores are dormant alternate life forms produced by the
Formation Of Endospores
Under conditions of starvation, especially the lack of carbon
endospore.
Endospore Structure:
The completed endospore consists of multiple layers of
and lipids
vegetative cell
environment.
the cell.
References
http://www.merck.com/media/mmhe2/figures/MMHE_17_190_01_eps.gif
http://www.microbiologytext.com/index.php?module=Book&func=displayart
icle&art_id=70
http://www.arn.org/docs/mm/flag_labels.jpg
http://answers.yahoo.com/question/index?qid=20090420112848AATic8k