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Dimapilis, Almira
ALT
200 ul
1000 ul
37 C
100 ul
1000 ul
ALT
Enzyme reagent
Starting reagent
-ketoglutarate - 15 mmol/L
Lactate Dehydrogenase
An enzyme that catalyzes the conversion of lactate
to pyruvate
Some of the organs relatively rich in LDH are the
heart, kidney, liver, and muscle.
Malate Dehydrogenase
Enzyme in the citric acid cycle that catalyzes the
conversion of malate into oxaloacetate.
Tris Buffer
Commonly known as
trishydroxymethylaminomethane
2-amino-2-hydroxymethyl-1,3-propanediol
Effective pH range between 6.5 and 9.7.
An effective buffer for slightly basic solutions.
L-aspartate
Classified as an acidic amino acid.
Non-essential- produced from oxaloacetate by
transamination.
Carries reducing equivalents in the malateaspartate shuttle, which utilizes the ready
interconversion of aspartate and oxaloacetate,
which is the oxidized (dehydrogenated)
derivative of malic acid.
NADH
in metabolism, it is used as a reducing agent
that donate electrons
The main role of NAD(P)+ in metabolism is
the transfer of electrons from one redox
reaction to another.
The energy is transferred to NAD+ by
reducing this to NADH, as part of glycolysis
and the citric acid cycle.
-Ketoglutarate
Keto acid produced by deamination of glutamate
Key intermediate in the Krebs cycle, coming
after isocitrate and before succinyl CoA
Anaplerotic reactions can replenish the cycle at
this juncture by synthesizing Alpha-ketoglutarate
Absorbance
0.017
0.011
0.005
Absorbance
0.017
0.007
0.005
Absorbance
0.031
0.041
0.047
Absorbance
0.228
0.213
0.194
Formula:
Hg 334nm
340 nm
Hg 365
971
952
1765
1780
1745
3235
SGOT:
1st sample
sample1 = A/min x 952
sample1 = [(a1-a2)+(a2-a3)]/2 x 952
sample1 = [(0.017-0.011)+(0.011-0.005)]/2 x 952
sample1 = 5.71 U/l
SGOT:
2nd sample
sample2 = A/min x 952
sample2 = [(a1-a2)+(a2-a3)]/2 x 952
sample2 = [(0.017-0.007)+(0.007-0.005)]/2 x 952
sample2 = 5.71 U/l
Increased SGOT:
Acute pancreatitis
Muscle dystrophy
Myocardial infarction
Hepatocellular disorder
SGPT:
1st sample
sample1 = A/min x 952
sample1 = [(a1-a2)+(a2-a3)]/2 x 952
sample1 = [(0.031-0.041)+(0.041-0.047)]/2 x 952
sample1 = 7.62 U/l
NV: Male = up to 18 U/l
Female = up to 15 U/l
Interpretation: Within normal values
SGPT:
2nd sample
sample2 = A/min x 952
sample2 = [(a1-a2)+(a2-a3)]/2 x 952
sample2 = [(0.228-0.213)+(0.213-0.194)]/2 x 952
sample2 = 16.18 U/l
NV: Male = up to 18 U/l
Female = up to 15 U/l
Interpretation: Slightly elevated due to
hemolysis
Increased ALT:
Viral hepatitis
Obstructive jaundice
Heart failure
Muscular dystrophy