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Antioxidants
Presented by
Yshteiaq M. Subhi.
Anantioxidantisamolecule
capableofinhibiting
theoxidationofothermolecules.
Oxidationisachemical
reactionthattransferselectronsor
hydrogenfromasubstanceto
anoxidizingagent.
Oxidationreactionscan
producefreeradicals.Inturn,
theseradicalscanstartchain
reactions.
Types of FreeRadicals
4. Hydrogen peroxide
6. Nitric oxide
7. Alkoxyl radical
8. Peroxynitrite
ROS Generation
Types of antioxidants:
Mainly Hydrophilic and Hydrophobic
Antioxidant enzymes:
1. Catalase
2. Glutathione peroxidase
3. Glutathione reductase
4. Super oxide dismutase (both Cu-Zn and Mn)
2. Ferritin
3. Lactoferrin
4. Metallotheinein
5. Transferrin
6. Hemoglobin
7.Myoglobin
Commonantioxidants(scavengers)
1. Bilirubin
2. Carotenoids
a. Beta-carotene
c. Beta-cryptoxanthin
e. Zeaxanthin
b. Alpha-carotene
d. Lutein
f. Lycopene
3. Flavonoids
a. Quercetin
b. Rutin
c. Catechin
4. Uric acids
5. Thiols (R-SH)
6. Coenzyme Q10
7. Vitamin A, C, E, D.
Others antioxidants
1. Copper
2.glutathione(GSH)
3. Alpha lipoic acid
4.Manganise
5. Selenium
6. Zinc
Mechanism of action
of antioxidants
Alpha tocopherol (vitamin E):
Beta Carotene
Ability to quench singlet oxygen, scavenge free radicals and protect
the cell membrane lipids from the harmful effects of oxidative
degradation .
Pro-oxidant activities
Antioxidants that are reducing agents can also act as prooxidants.
For example, vitamin C has antioxidant activity when it reduces
oxidizing substances such as hydrogen peroxide, however, it will
also reduce metal ions that generate free radicals through
theFenton reaction.
2 Fe3++ Ascorbate 2 Fe2++ Dehydroascorbate
2 Fe2++ 2 H2O2 2 Fe3++ 2 OH+ 2 OH
Determining
Antioxidant Activity
ORAC, Oxygen Radical Absorbance Capacity method
TRAP, Total Radical-Trapping Antioxidant Parameter method.
TEAC,
Trolox
Equivalent
Antioxidant
Oxyradical
Scavenging
Capacity method
DPPH
TOSC,
Total
Capacity method
PSC, Peroxyl Radical Scavenging Capacity
method
FRAP, Ferric Reducing/Antioxidant Power method.
Health effects:
Disease
treatment
Disease
prevention
Physical
exercise
Adverse
effects
Benefits of antioxidants:
Destroythefreeradicalsthatdamagecells.
Promotethegrowthofhealthycells.
Protectcellsagainstpremature,abnormalaging.
Helpfightage-relatedmaculardegeneration.
Provideexcellentsupportforthebodysimmunesystem,makingit
aneffectivediseasepreventative.
No Matter What Your AgeYou Need Antioxidants!
grain cereals, eggs, meat, legumes and nuts. Some antioxidants such
aslycopeneand ascorbic acid can be destroyed by long-term storage or prolonged
cooking.Other antioxidant compounds are more stable, such as the polyphenolic
antioxidants in foods such as whole-wheat cereals and tea.
Natural antioxidants
Antioxidant compounds
VitaminC(ascorbicacid)
FreshFruitsandvegetables
Vitamin
E
tocotrienols)
(tocopherols,
Vegetableoils
Tea,coffee,soy,fruit,olive
Polyphenolic
antioxidants
oil,chocolate,cinnamon,orega
(resveratrol,flavonoids)
noandredwine
Carotenoids(lycopene,
carotenes,lutein)
Fruit,vegetablesandeggs.
synthetic antioxidants
Propylgallate(PG,E310),
Tertiarybutylhydroquinone(TBHQ),
Butylated hydroxyanisole (BHA,E320)
Butylated hydroxytoluene (BHT, E321).
Uses in technology:
Food preservatives
Industrial uses
Conclusion
Antioxidant plays an important role to prevent cancer, and other disease.
They also have role in slowing ageing process and preventing heart
disease. So antioxidant are very much necessary for our body .But our
body cant manufacture these chemicals, so they must be supplied
through diet.
ANY QUESTIONS