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Section Six1
Interferences
Interferences that are expected:
Physical
Matrix
Spectral
Lead to incorrect results
Physical Interference
Common in ICP emission and ICPMS
Sample matrix effects
Surface tension
Viscosity
Matrix Interferences
Affect ion intensity in two different ways
Plasma Loading
Space Charge Effect
Readings are generally suppressed
Routinely compensated for by internal
standardization
Plasma Loading
Energy reduced in plasma
Minimized by:
Keep dissolved solids below 0.5%
Robust plasma conditions
High RF power
Lower Nebulizer flow
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Cylinder Lens
(Net + Charge on Lens)
Skimmer
Internal Standards
Internal Standard Selection
Low concentration or not present in
samples
Not interfered by sample matrix
Do not interfere on analytes
Not common environmental contaminants
Matched to mass(es) of analytes
Reasonable concentrations
Internal Standards
Frequently Employed Internal Standards
On Line Setup
B019-3342
0990-8587
Black/Black
B050-1595
B019-1058
Mixing Block
B050-7962
B019-3873
Pt/Ir Female
Connector
B019-3342
Hose
Adaptor
Tubing Size
Dependant on
Dilution
Internal
Standard
Capillary
0990-8265
To Nebulizer
To Autosampler
Probe
B019-1059
On Line Setup
Internal Standards Kits
Non HF N0774068
HF N0774067
Does not include
Nebulizer
Pump Tubing
Pump
Element
46
Ca
0.004
Ti
8.0
47
48
49
50
5.5
5.4
0.187
7.3
73.8
Isobaric Correction
Counts at mass 114 = Cd 114 + Sn 114
or
Isobaric Correction
Write element equations for the following isotopes:
35 + 16 = 51
40 + 35 = 75
32 + 16 = 48
Organic matrices
CO on 28Si
ArC on 52Cr
12 + 16 = 28
12 + 40 = 52
40 + 16 = 56
98 + 16 = 114
Corrections
Isotope Selection
High abundance isotopes for low concentration
elements
Lower abundance isotopes for high concentration
elements
Avoid isotopes with intense background
contributions
Use 54Fe or 57Fe instead of 56Fe to avoid 40Ar16O
Corrections
Inter-element corrections
Dynamic
Empirical
DRC
Covered later
Minimize water vapor
Different sample introduction
Separation
Hydride
Polyatomic Correction
Dynamic Correction
Determine interfering molecular ion
intensity at an alternative mass.
Example:35Cl16O interference on 51V
V = Intensity51 - a35Cl/a37Cl* Intensity53
Advantages:
Works on wide Cl concentration ranges
Not affected by change in plasma
As in monoisotopic at 75amu
40Ar35Cl can cause erroneously high results
Measure the 40Ar35Cl contribution and subtract it from the total
counts at mass 75
Counts mass 75 = 75As counts + 40Ar35Cl counts
or
75As = mass 75 - 40Ar35Cl
500 mg/l Cl
Sample
i1
i3
35
Cl16O
i4
i2
i51V
35
51
35
i51V = I4 -
51
i2
i1
* i3
35
51
Interference Summary