BRAIN LIPID

EXTRACTION
Group 3
Basmillo|Borreta|Beltran

OBJECTIVES
To

be able to extract the

lipids present in the pigs
brain amd characterize them:
Specifically on:
Fatty Acids, Lecithin and
Cholesterol

OBJECTIVES
To

be able to characterize the

different properties of soap
and glycerol

SAPONIFICATION

3KOH

Test on SOAP: Salting

Out

TEST ON SOAP: Salting Out

EXPERIMENTAL
RESULTS

White
precipitate
soluble in
water

THEORETICAL
RESULTS

White
precipitate
soluble in
water

TEST ON SOAP: Insoluble

Salts
EXPERIMENTAL
RESULTS

White
precipitate
insoluble in
water

THEORETICAL
RESULTS

White
precipitate
insoluble in
water

SURFACE TENSION
The

force
with which
surface
molecules of
a liquid are
held together

Role of Surface Tension

It

leads to efficient absorption

> Enzymatic Reactions
> Formation of complex
compounds
> Action of drugs and poisons

Gibbs-Thomsons
principle
Solutes, dispersing in a liquid
instead of concentrating on its
surface, raise its surface tension
while those concentrating on the
liquid surface lowers its surface
tension.

SURFACE TENSION TEST

EXPERIMENTAL
The
sulfur
RESULTS

powder sank
in the soap
solution and
stayed afloat
in water and
2% NaCl
solution

THEORETICAL
RESULTS
The sulfur powder
will:
SINK in SOAP
SOLUTION
FLOAT in WATER
and 2% NaCl

Application
Practical
Application:

Hays test for

bile salts in
urine

STRUCTURE OF FATTY
ACIDS

NON
POLAR

TEST ON FATTY ACIDS:

Solubility
THEORETICAL
RESULTS

INSOLUBLE in
WATER
SOLUBLE in
CHCl3

TEST ON FATTY ACIDS:

Translucent Spot Test

TEST ON FATTY ACIDS:

Translucent Spot Test
EXPERIMENTAL RESULTS

Presence of
translucent spot
that remains
even after long
standing

THEORETICAL RESULTS

Presence of
translucent spot
that remains
even after long
standing

TEST ON FATTY ACIDS: Iodine

Absorption Test
Mechanism:

TEST ON FATTY ACIDS: Iodine

Absorption Test
EXPERIMENTAL RESULTS

THEORETICAL RESULTS

Decolorization
of iodine
solution

Decolorization
of iodine
solution

GLYCEROL

TEST ON GLYCEROL:
Solubility
EXPERIMENTAL RESULTS

SOLUBLE in
WATER
INSOLUBLE in
CHCl3

THEORETICAL RESULTS

SOLUBLE in
WATER
INSOLUBLE in
CHCl3

TEST ON GLYCEROL: Acrolein

Test
Reaction:

TEST ON GLYCEROL: Acrolein

Test
EXPERIMENTAL RESULTS

THEORETICAL RESULTS

Pungent odor

Pungent odor

TEST ON GLYCEROL:
Translucent Spot Test
EXPERIMENTAL RESULTS

Presence of
translucent
spot which
does not fade
over time

THEORETICAL RESULTS

Presence of
translucent
spot which
does not fade
over time

Benedicts Test

Test

for the presence of reducing sugars

TEST ON GLYCEROL:
Benedicts Test
EXPERIMENTAL RESULTS

THEORETICAL RESULTS

Positive
Positive

LECITHIN

LECITHIN
Aka

phosphatidylc
holines
Most abundant
phospholipids
of the cells
membrane
Amphipathic

FUNCTIONS
Lung

surfactant
Component of cell membranes
Forms protective layer in the
membrane for proper neuron
transmission

STRUCTURE

TEST ON LECITHIN: Acrolein

Test
EXPERIMENTAL RESULTS

THEORETICAL RESULTS

Pungent odor
Pungent odor

TEST ON LECITHIN: IodineAbsorption Test

EXPERIMENTAL RESULTS

Decolorization
of iodine
solution

THEORETICAL RESULTS

Decolorization
of iodine
solution

TEST ON LECITHIN: For

Phosphorus

TEST ON LECITHIN: For

Phosphorus
EXPERIMENTAL RESULTS

THEORETICAL RESULTS

Formation of
yellow
precipitate

Formation of
yellow
precipitate

Formation of
crystals upon
cooling

Formation of
crystals upon
cooling

ISOLATION AND TESTS

ON CHOLESTEROL

CHOLESTEROL

Precursor

of some hormones,
vitamins, steroids

CHOLESTEROL:
CRYSTALS

TEST ON CHOLESTEROL:
Salowski Test

TEST ON CHOLESTEROL:
Salowski Test

TEST ON CHOLESTEROL:
Salowski Test
THEORETICAL
RESULTS

CHCl3 layer:
Rose red color
Acid layer:
Yellowish with
Fluorescent
green color

Liebermann-Burchard Test
reaction

TEST ON CHOLESTEROL:
Liebermann-Burchard Test
THEORETICAL RESULTS

Green solution