The 60th Annual General Assembly and Scientific Meeting of

the Japan College of Rheumatology

Oral Session

Curcumin Prevented the Progressivity of

Nephritis in Pristane Induced Lupus Mice
Tita Luthfia Sari, Mirza Zaka Pratama, Cesarius Singgih Wahono, Handono Kalim

Rheumatology and Immunology Division Department of Internal Medicine and

Department of Clinical Pathology

Faculty of Medicine Brawijaya University, Indonesia

Existence of conflict of interest : Non

In relation to this subject, there are no companies or for-profit organizations which have a COI relation.

Introduction
According to several study, turmeric's main compound,
known as curcumin, has shown potential in modulating
of immune response
Modulation of the immune system are currently being
developed for the treatment of systemic lupus
erythematosus (SLE)
Renal complication is found in most patients with SLE,
and the main goal of therapy is to prevent the
progressive loss of renal function

Methods
Pristane-induce
Lupus

Twenty four female Balb/c mice were given single

injection of 0.5 ml pristane intraperitoneally to induce
lupus manifestations

Curcumin

16th weeks after pristane injection, mice were devided

into 4 groups:
- control group (no curcumin)
- group 1 (12.5 mg/kgBW/day curcumin)
- group 2 (50 mg/kgBW/day curcumin)
- group 3 (200 mg/kgBW/day curcumin)
Curcumin were given orally every day for 16 weeks

Results

Proteinuria
Histopathological analyses of the kidney, including
classification, activity index, and chronicity index of
lupus nephritis

Pristane Induced
Lupus Mice
Observed for 16 weeks

Control Group

Group 1

Group 2

Group 3

12.5mg/kgW/day
curcumin

50mg/kgW/day
curcumin

200mg/kgB/day
curcumin

Curcumin was given orally for 16 weeks

Evaluation of proteinuria and
renal histopathology

Results
Clinical Manifestation of
Pristane-induce Lupus Mice
Manifestation

Rash

5/24 (20.8%)

Arthritis

18/24 (75%)

Proteinuria

10/24 (41.7%)

Ascites

5/24 (20.8%)

Alopesia

2/24 (8.3%)

Evidence of Proteinuria and Histopathology

Analysis of Nephritis Lupus
Control
Group

Group 1

Group 2

Group 3

5/6 (83.3%)

4/6 (66.6%)

1/6 (16.6%)*

0/6 (0%)***

0/6(0%)

0/6 (100%)

0/6 (0%)

3/6 (50%)

Class II

0/6 (0%)

1/6 (16.6%)

2/6 (33.3%)

3/6 (50%)

Class III

2/6 (33.3%)

1/6 (16.6%)

1/6 (16.6%)

0/6 (0%)

Class IV

4/6 (66.6%)

4/6 (66.6%)

3/6 (50%)

0/6 (0%)

Class V

0/6 (0%)
0/6 (0%)

0/6 (0%)
0/6 (0%)

0/6 (0%)
0/6 (0%)

0/6 (0%)
0/6 (0%)

Activity Index

6.33 0.615

5.83 0.872

1.67 0.333***

Chronicity Index

1.17 0.477

1.33 0.760

4.33 0.760
1.33 0.494

Proteinuria
Class I

Class VI

* p< 0.05, ***p<0.001 compared to control group

0.50 0.224

Histopathology Analysis of Kidney

LI

: Leucocyte infiltrate

IF

: Interstitial fibrosis

MH : Mesangeal hyperproliferation
NM : Normal mesangeal
GS : Glomerular sclerosis
Control Group

Group 1

Group 2

Group 3

Activity and Chronicity Index of Nephritis Lupus

Conclusion
Curcumin is promising natural products that should be
extensively investigated as complementary theraphy
for SLE

The 60th Annual General Assembly and Scientific Meeting of

the Japan College of Rheumatology
Yokohama, 21-23th April 2016

Thank
You...