CELL ISOLATION

GROUP 7:
YUDI DUWI PERDANA (1510424010)
YELSHA RAMADHILA (1510424011)
FITRA ALZAKIA ASYARI (1510424013)

CELL ISOLATION

DEFINITION

SECTION

DEFINITION
isolation cell is a cell-taking
process of a particle from its
original location for further
observation.

SECTION

DNA ISOLATION

RNA ISOLATION

PROTEIN ISOLATION

DNA ISOLATION
PURPOSE : to separating DNA from
another subsatnce like protein, fat, and
carbohydrat.
PRINCIPLE :

- Lysis (destruction)
- Extraction (separation)
- DNA purification

step how to isolating DNA

destruction the cell walls, usually uses
liquid nitrogen where temperatures -169C.
Lysis of the cell membrane is the process in
order to remove the cell membrane to the
nucleus.
Purification (purification) DNA aims to
remove some contaminants such as
secondary compounds (phenol),
polysaccharides, RNA and protein.

RNA ISOLATION
PRINCIPLE
warning RNA handling
sample preparation
isolation
check out quantifying and quality
purification.

step how to isolating RNA

In general, the handling of DNA isolation is in all
its activities using gloves.
Samples coming from the field should be
washed first and extract RNA stored in freezer
-80.
Stages of DNA isolation using the GTC method.
Stages isolation GTC method consists of lysis,
separation, leaching and purification of RNA and
presiptasi.
Separation
cleanig

PROTEIN ISOLATION
purpose
: to study the characters and
function of proteins.
types:
1. Dialysis way
2. Cells Filtration Chromatography way
3. Ion Exchange Chromatography way