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Reaction coordinate
2007 Paul Billiet ODWS
The Lock and Key Hypothesis
This explains enzyme specificity
This explains the loss of activity when
enzymes denature
Reaction
velocity
Substrate concentration
Vmax
Reaction
velocity
Substrate concentration
Faster reaction but it reaches a saturation point when all the
enzyme molecules are occupied.
If you alter the concentration of the enzyme then Vmax will
change too.
2007 Paul Billiet ODWS
The effect of pH
Optimum pH values
Enzyme
activity Trypsin
Pepsin
1 3 5 7 9 11
Q10 Denaturation
Enzyme
activity
0 10 20 30 40 50
Temperature / C
CH2
CH2COOH CHCOOH
COOH
Malonate