DNA extraction and after centrifugation the pellet reformed. -What is solution(A) and Pellet(B)? Question2 What the function of the following:- -Glycerol -Pico dye Question3 Justify:-Presence of freezers with different temperature (4,-20,-40 and -80 C ) in molecular labs? Question4 What is a DNA pellet? How can one define this term logically and scientifically? *This refers to the mass of DNA at the bottom of the centrifuge tube after you do a DNA extraction. *DNA is insoluble in ethanol, so when you add ethanol to a mixture containing DNA, the DNA will precipitate in the ethanol. Centrifuging will leave a 'pellet' of DNA.
*A DNA pellet is a clump of DNA that has been
precipitated from an aquaeous solution by the addition of alcohol. PCRmaster A PCR master mix is a mix of all most of the ingredients or factors necessary for PCR to occur. The goal of a PCR master mix is to decrease time spent in pipetting.