Professional Documents
Culture Documents
DESIGN BIOREACTOR
FOR THE
FOR THE PRODUCTION
PRODUCTION
OF
OF
BIOPHARMACEUTICALS
BIOPHARMACEUTICALS
FROM ANIMAL
FROM ANIMAL CELLS
CELLS
06/07/17
CHE 654
BIOREACTOR ENGINEERING
EH242 (5A)
14 DECEMBER 2016
IR. PN NORMADYZAH BT
PRESENTED BY:
(2014880054)
(2014671946)
Airlift bioreactor
Impeller Spacing:
-The spacing between impellers should be 1.0di to 2.0di , where di is the diameter
of the impeller.
-Proper spacing between impellers should be Di < Hi < 2Di
Baffling:
-Stirred tank fermenters generally use baffles because of the need to disrupt the
bulk fluid flow in the tank.
-4 baffles if the tank diameter less than 3m
-6-8 baffles if the tank diameter larger than 3m
-Width of baffles, Dt/10 and Dt/12
-Diameter of vessels baffle 10< Dt/Db< 12
Tank Height:
-The height to diameter ratio of the tank is typically between 2.0 and 3.0; however,
taller tanks (up to HL/dt=4.0) have been used to reduce the power requirement of
the impellers.
-Diameter ratio between 2:1 to 3:1
-If H=D, one agitator is enough
-If H=2D or more, additional set of agitator should be added.
Volume:
-75% of the total vessel volume is filled with liquid
Standard geometrical Dimension of STR
STIRRED-TANK BIOREACTOR
Advantages Disadvantages
Low operating cost Foaming is often a problem but
it is overcome by using proper
antifoaming agents
Low investment need Size limitation by motor size,
shaft length and weight
Continuous operation
Easy to clean
Simplicity of construction
Good temperature control
Easily adapts to two phase runs
AIRLIFT FERMENTORS
Air driven
External and internal loop system
Used in a number of large-scale processes
pH control
For small scale operation, can maintain pH by using gaseous CO2.
The acid is added by sparging carbon dioxide from the bottom of the
reactor. As the CO2 bubbles its way to the top, it forms carbonic acid
decreasing the pH. The base is added with carbonate as a fluid.
For large scale cultures, can directly add acid or base
How?
insert probe into culture to detect change of pH
then the cell will experience a very similar environment.
2. Diaphragm gauge
3. Piezoelectric gauge
Foam Formation
The appearance of foam is very undesirable phenomenon, since there
is a risk to lose an essential part of medium broth.
During foaming, it is not possible to perform high quality analysis and
measurements.
How to eliminate foam formation?:
Addition metering of antifoam based on sensor
Probe is inserted through top of bioreactor, stainless steel rod set at a
defined level above broth surface.
When foam rises and touches the probe tip, pump is activated and
antifoam is released into bioreactor
the jacket surrounding the bioreactor and through the use of heat
exchangers
system
medium exchange
DISSOLVED OXYGEN
Cell culture require oxygen for production of energy from organic carbon sources (eg, glucose)
The control of oxygen flow is needed to ensure it does not become a rate-limiting factor in process.
High DO level can increase reactive oxygen species causing oxidative damage to cells and products.
cellular metabolism
product quality
06/07/17