Engineering Dr. Sherri Clark / The National Biotechnology Information Facility
The World of Microbes
on the Internet This presentation 1998 by the Regents of New Mexico State University Topi cs of Discussion DNA History What is it? Genetic Engineering? Examples of uses for animals. Examples of uses for humans. Examples of uses for plants. Cloning Ethical Questions DNA History Until early to mid 1900s a majority of biologists believed that proteins had to be the genetic material. 1928 - Griffith showed evidence of bacterial transformation (uptake of genetic material). 1944 - Avery and coworkers showed that this transforming agent was DNA. DNA History 2 1952 - Hershey and Chase determined that DNA was also the agent that viruses like T4 injected into host cells for reproduction. DNA History 3 1953 - Watson and Crick described the structure of DNA. 1961 - Crick described the genetic code as triplet codons. What is DNA? Macromolecule Polymer Phosphate, ribose sugars, with purine and pyrimidine bases. Anti-parallel strands. What is DNA? 2 Double helix.
Copyright 1994-1998 Institute for Molecular Virology.
University of Wisconsin - Madison. What is DNA? 3 Carries genetic information. The information language is made up of words called genes and letters called codons. Genetic Engineering Also known as recombinant DNA technology. The manipulation of an organisms genetic material, DNA or sometimes RNA, to produce a desired gene product or characteristic. The end product of these techniques is often a chimeric organism. Definitions Chimera Cloning Vector Restriction Endonuclease Methylase Sticky Ends Blunt Ends Cloning a Gene Into E. coli Cloning a Gene Into E. coli 2 Examples of Uses for Animals Cows and Pigs. Bovine Somatotropin Porcine Somatotropin Examples of Uses for Humans Humans insulin and growth hormone. -lactamases and antibiotics. gene therapy. Examples of Uses for Plants Examples of Uses for Plants 2 Potato Examples of Uses for Plants 3 Tomato
Corn and Cotton.
Cloning Making an organism with a genetic makeup that is identical to another organism.