Baca absorbansi lagi setelah 1 menit, 2 menit, dan 3 menit
ASAT activity (U/L)= A/min sample x 1745
DISKUSI Apa yang anda ketahui tentang AST? Apakah fungsi AST bagi metabolisme didalam tubuh? Mengapa AST bukan indikator yang spesifik bagi Liver? REAGENT COMPOSITION R1:1. TRIS (pH=7,65) 110 mmol/L 2. L-Aspartate 320 mmol/L 3. Malate Dehydrogenase (MDH) 800 U/L 4. Lactate Dehydrogenase (LDH) 1200 U/L R2:1. 2-Oxoglutarate 65 mmol/L 2. NADH 1 mmol/L TEST PRINCIPLE 2-Oxoglutarate L-Aspartate
AST/GOT
L-Glutamate Oxaloacetate NADH+ H+
MDH
L-Malate NAD+
NADH is oxidized to NAD+, the resulting decrease in absorbance at 340
nm is directly proportional to the activity of GOT in the sample. This is a modified formulation for the assay of GOT, as recommended by the IFCC (International Federation of Clinical Chemistry). CALCULATION GOT (IU/L) = A/min x Factor Substrate Start: 25 or 30C 37C Factor at 340 nm 1151 2143 Factor at 334 nm 1173 2184 Factor at 365 nm 2132 3971 Sample Start: 25 or 30C 37C Factor at 340 nm 952 1745 Factor at 334 nm 971 1780 Factor at 365 nm 1765 3235 REFERENCE RANGE *(IU/L)
25C 30C 37C
males <18 <25 <37
females <15 <21 <31
Aspartate Transaminase (SGOT) AST is called serum glutamic oxaloacetic transaminase (SGOT). Aspartate aminotransferase (AST, EC 2.6.1.1) is enzymes found mainly in the liver, but also found in red blood cells, heart cells, muscle tissue and other organs, such as the pancreas and kidneys. 1 = Malate dehydrogenase 2 = Aspartate aminotransferase Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and -ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal 1 = Malate disease and trauma. dehydrogenase 2 = Aspartate aminotransferase 1 = Malate dehydrogenase 2 = Aspartate aminotransferase
REVIEW KEBIJAKAN TENTANG PELAYANAN KESEHATAN PUSKESMAS DI DAERAH TERPENCIL PERBATASAN (Policy Review On Health Services in Primary Health Center in The Border and Remote Area)