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• Introduction.
• Principle of HPTLC.
• Difference between TLC & HPTLC.
• Steps involved in HPTLC.
• Material used for plates.
• Mobile phase.
• Sample application.
• HPTLC Plate development.
• Applications of HPTLC.
• Sophisticated form of thin layer
chromatography. It involves the same
theoretical principle of thin layer
chromatography.
• Traditional Thin Layer Chromatography & its
modern instrumental quantitative analysis
version HPTLC are very popular for many reasons
such as
o visual chromatogram,
o simplicity,
o multiple sample handling,
o low running and maintenance costs, disposable layer
etc.
Separation may result due to adsorption
or partition or by both phenomenon
depending upon the nature of adsorbents
used on plates and solvents system used
for development.
HPTLC TLC
Chromatography development
Detection of spots