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Amino acid
Amino Acids Are Molecules Containing An Amine Group, A Carboxylic
Acid Group And A Side-chain That Varies Between Different Amino
Acids. The Key Elements Of An Amino Acid Are Carbon , Hydrogen ,
Oxygen , And Nitrogen An Alpha-amino Acid Has The Generic
Formula H2NCHRCOOH, Where R Is An Organic Substituent. Amino
Acids Are Critical To Life, And Have Many Functions In Metabolism.
One Particularly Important Function Is To Serve As The Building Blocks
Of Proteins , Which Are Linear Chains Of Amino Acids. Amino Acids
Can Be Linked Together In Varying Sequences To Form A Vast Variety
Of Proteins.
Essential and Nonessential AAs
Commercial Production
Glutamic acid > lysine > methionine > threonine > Aspartic acid
CHEMICAL INDUSTRY
3. MICROBIOLOGICAL SYNTHESIS
a. Direct fermentation: MO use carbon sources and produce aa.
Carbon like glu, fructose, alkanes, ethanol, glycerol, molasses,
starch, methanol etc.
b. Conversion of metabolic intermediates to amino acids:
c. Use of enzymes (microbial) or immobilized cells: resting cells,
crude cell extracts, immobilized cells can be used.
STRAIN IMPROVEMENT METHODS FOR AA PRODUCTION
Regulatory control:
1. Carbon sources
2. Nitrogen source: ammonia for carbon to glu pH control
3. Growth factors :biotin
4. O2 supply: high conc inhibits growth and low O2 leads to lactic
acid production and succinic acid, Afftects Glu production in both
cases
Nutrients 38oC
Dissolving tank 30-35h
Glucose (12%)
Sterlizer
Buffer tank
Cell separator
Eluted in NaOH
Anion exchanger
Evaporation
Crystallization
100g/L
Bakteri asam laktat juga memproduksi asam format, asetat,
propionat, kaproat, kaprilat, kaprat, butirat, dan isovalerat dari
metabolisme fermentasi dan transformasi enzimatik asam amino.
Protease merupakan enzim yang mendegradasi protein. Protease
tergolong enzim hidrolase yang mengkatalisis reaksi degradasi
substrat dengan pengolahan air. Pengertian protease menurut
Winarno (1985) ada dua yaitu proteinase yang mengkatalisis
reaksi hidrolisis molekul protein menjadi fragmen besar polipeptida,
dan peptidase yang menghidrolisis fragmen besar polipeptida
menjadi asam amino.
Kasein pada yogurt sebagai substrat untuk uji aktivitas protease
dengan metode Lowry. Kasein akan terhidrolisis menjadi peptida
dan asam amino dengan bantuan air oleh enzim protease.