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LABORATORIO
Encender los equipos a utlizar:
baños maria, platos calientes,
destilador de agua.
Limpiar el area a utilizar
Buscar hielo
Descongelar los reactivos en el
hielo.
Identificar el procedimiento o
revisar el protocolo
Identificar todos los reactivos y las
formas seguras de trabajar: batas,
guantes, gafas o mascaras de
proteccion.
Preparar las soluciones necesarias
Iniciar el proceso
La seguridad es crítica en el desarrollo de
ciencia sostenible de clase mundial
2
3
Tails
The cystic fibrosis transmembrane conductance regulator (CFTR) gene contains 250,000 base pairs.
In the majority of cystic fibrosis patients, three base pairs are missing. This leads to the loss of a
single amino acid from the 1,480 residues in the protein...and this change ultimately kills a person.
DNA,
PROTEINS
HAVE
ELECTRICAL
CHARGE
DNA - template
dNTP’s: deoxynucleotides
Polymerase
Buffer solution for enzyme activity Mg2
Primer sequences
Thermocyclers
CCD Panel
Size Ar+
Separation LASER
(488 nm)
Detection Color
region Separation
More Sensitive
Less Risk
Better Crop
Domestication Project for SPF Brooder in Taiwan
Control Systems of IQ2000 System
Basic criteria for a accurate PCR result: good extraction,
successful PCR reaction,
no contamination
Data of IQ2000 WSSV System
1: sample with severe WSSV infection
1 2 3 4 5 6 7 8 9 M 2: sample with medium WSSV infection
3: sample with light WSSV infection
4: sample with very light WSSV infection
5: WSSV negative sample
6: ddH2O
7: Standard 1, 1000 copies/reaction
8: Standard 2, 100 copies/reaction
Confirm that there is no 9: Standard 3, 10 copies/reaction
contamination during M:molecular weight marker
PCR procedure
Diadema mexicanum
ECHINOMETRA
ATLANTICO PACIFICO
Diadema mexicanum
ECHINOMETRA
ATLANTICO PACIFICO
Anisotremus
Lutjanus
TERAPIA DE CÉLULAS MADRE
DÍA 4
Cavidad
Masa de células
internas
células
(células Madre
de
Masa de células Embrionarias)
cubierta
internas
interna
Células
Células NERVIOSAS Formadoras de
SANGRE
CELULAS MADRE
BLASTOCISTO
Modificado de: Scientific American, 2001 Células PANCREATICAS Células CARDIACAS
To analyze the data that follows we need only consider the
genotypes of the parents and their offspring. The alleles are
inherited as describe by Mendel.
Diploid parents each pass on one of their two alleles to their
offspring according.
You will have 2 allelles per loci involve to compare and
answer our questions.
Quien es el hijo de esta pareja
RATON
GATO
Aún hay mucho más que descubrir
Y ustedes pueden ser sus descubridores…
hay tan solo que observar, cuestionarse,
encontrar respuestas verdaderas y sugerir
soluciones…
Les agradezco su atención y participación
y los espero en STRI…