SNaPSHot

NAME ID
NUR LISSA BT RAMELI 1134150714835
NUR AISYAH BT AYUB 1134150714838
LAW SEI YING 1134150714848
NUR NABILAH BT ABDULLAH 1134150714840
 SNaPSHot

• Function: is a primer extension–

based method developed for the
analysis of single nucleotide
polymorphisms (SNPs)
Work flow of SNaPSHot
 Firstly, the primers were The primer binds to a complementary
used to amplify the target template in the presence of
SNPs fragment. fluorescently labeled ddNTPs and DNA
polymerase.

The polymerase extends the

Then, the corresponding SNP loci
primer by one nucleotide,
were determined according to the
adding a single ddNTP to its 3´
position and color of the peak.
end.

According to the color of the peak, we can

know the type of the base, and identify the
gene sequence of the sample.
 Advantages of SNaPSHot

• High accuracy
• Saving time and money
• Require little optimization
• High throughput level
• Consistence and easy to use
• Multiplex capability allows low cost
• Sensitive allele-frequency detection
• Automated analysis
 References

• https://www.cd-genomics.com/snapshot.html

• https://assets.thermofisher.com/TFS-

Assets/LSG/brochures/cms_101014.pdf