SPECTROSCOPY (AES) Devon Brown SUPA Forensics Block 4 What Is AES?
• A method of chemical analysis that measures the
intensity of light emitted by the atoms of elements at specific wavelength Chemical Principles • Electrons from a higher energy level drop to a lower energy level • Electron emits a photon of light to dispose of extra energy How it Works • Flame, spark, or plasma excites the electrons • Electrons jump to a higher energy level • As electrons return to ground state, they release a photon • Instrument measures intensity of photons and their wavelength • Determines elements present and they’re quantity Emission Lines • The frequency of an atom moving from a high energy level to a lower energy level • Wavelengths of photons emitted create lines • Each element has unique emission lines • Qualifies elements present in sample Emission Band • Graph of the intensity of the light emitted from a sample • Intensity is a function of the wavelength • Separates various elements by color (like emission lines) • Quantifies the amount of an element in a sample Flame Atomic Emission Spectroscopy • Sample is in the form of a sprayed solution or gas • Flame heat evaporates solvent and breaks chemical bonds • Produces free atoms of the material • Heat changes atoms into electrically charge particles • Emit light as they lose energy • Wavelength of light characteristic to specific element • Light dispersed by a prism and detected in spectrometer Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES) • Inductively coupled plasma produces excited ions • Ions and atoms radiate electromagnetically charged particles • Wavelengths produced correspond to a definite element Uses • Determine the proportional quantity of a particular element in a given sample • Determine the elemental composition of an unknown sample • Study structures of atoms • Analyzing motor oils • Determine the presence of arsenic in food and metals in wine • Study trace elements that are bound to proteins AES IN FORENSIC SCIENCE Uses • Analyze trace elements in soil • Determine origin • Determine the composition of metallic and glass samples • Determine origin and/or manufacturer • Confirm a poison • Detect a drug Evidence • Preparation: samples must be converted into highly excited free atoms • Solid samples (glass, soil, metal alloy) • Directly vaporize sample • Excite sample by using a laser pulse or spark between electrodes • Introduced to a exciting source by a semiliquid mixture or melted by a laser in a stream of gas • Liquid samples (bodily fluids, unknown solution) • Reduced to a fine spray • Carried to exciting source by flowing gas Safety and Hazard Issues • Chemicals tested can be very dangerous • Keep away any flammable materials • Extreme temperatures • Glass and metal samples can have sharp edges • Biological samples can contain poisonous materials and disease Difficulties/Interference • Self-absorption • Flame’s temperature is greatest at center – concentration of excited atoms greater at center • Atoms around outer edges not in the same state • Excited atom emits a photon – grounded atoms in outer regions of flame may absorb photon • Decrease the emission intensity Limitations Originally: • High possibility of chemical interaction • Need a fairly large sample • Sensitivity of instruments low • Instability of atomization source • Cannot conduct simultaneous or multi-elemental analyses
With the development of technology, most of these
limitations have been overcome. Advantages Disadvantages • Several elements can be recorded at once • Equipment is more • Higher temperatures means expensive lower inter-element • Procedure is more interference • Several elements can be complicated analyzed from a very small • More time consuming sample • Higher operating costs • Nonmetals can be determined by plasma • Low concentration refractory compounds can be determined • High concentration range for plasma sources Works Cited • “Atomic Spectroscopy.” Andor: An Oxford Instruments Company. www.andor.com. Web. March 29, 2015. • Banerjee, Prasenjit. “Atomic Emission Spectroscopy.” Chemistry Learner. www.chemistrylearner.com. Web. March 17, 2012. March 30, 2015. • Cylinder, Drew. “Atomic Spectra.” UC Davis Chemwiki. chemwiki.ucdavis.edu. Web. March 29, 2015 • Harvey, Davis. “Atomic Emission Spectroscopy.” UC Davis Chemwiki. chemwiki.ucdavis.edu. Web. March 29, 2015. • Rosen, Vasiliy, Ph.D. “Atomic Spectroscopy: Basic Principles and Instruments.” Slideshare. www.slideshare.net. Web. March 28, 2011. March 29, 2015. • Scheeline, Alexander and Spudich, Thomas M. “Atomic Emission Spectroscopy.” Analytical Science Digital Library. www.asdlib.org. Web. March 30, 2015. • Spencer, James T, Ph.D. An Introduction to Forensic Science: The Science of Criminalistics. Cengage Learning. 2007-2012. March 29, 2015 • Stone, David C, Ph.D. “Atomic Spectroscopy: Intrumentation, Techniques, and Theory.” Chemistry University of Toronto. www.chem.utoronto.ca. Web. May 7, 2012. March 29, 2015. • Troy, David B. The Science and Practice of Pharmacy. Remington. 2006. books.google.com. Web. March 30, 2015.