FIXATION &

FIXATIVES
Objectives of Fixation:
Two Mechanisms Involved in Fixation
Main Factors Involved in Fixation
Main Factors Involved in Fixation
Practical Considerations of Fixation
Factors That Affect Fixation
Effects of Fixatives in General
Characteristics of Good Fixatives
TYPES OF
FIXATIVES
According to COMPOSITION
According to ACTION
According to ACTION
Special Fixations
Special Fixations
 I. ALDEHYDE FIXATIVES

-
-
 II. METALLIC FIXATIVES

- excellent for Trichrome stain

- mercuric deposits are removed by immersing tissues in alcoholic

iodine solution. “de-zenkerization”
Su = sublimat (HgCl2)
Sa = saure (acid)
- fixes connective tissue mucin
III.PICRIC ACID FIXATIVES
IV. GLACIAL ACETIC ACID
V. ALCOHOL FIXATIVES
 VI. OSMIUM TETROXIDE ( OSMIC
ACID)

F.2
VII.TRICHLOROACETIC ACID
VIII.ACETONE
IX. HEAT FIXATION
Fixation Artifacts
MICROWAVE TECHNIQUE
 IMMUNOFLOURESCENCE &
IMMUNOPEROXIDASE TECHNIQUES