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ISOLATION OF MANGIFERIN:
Plant parts are subjected to deffating by petroleum ether and extraction by soxhlet apparatus with
ethanol.
Bhuvaneswari (2013) used the leaves, the concentrated mass were resuspended in ethanol
then partitioned with dichloromethane 4 times. The aqueous-alcoholic phase was treated with
H2 SO4 then partitioned with ethyl acetate for 3 times, dried, and dissolved in ethanol and
cooled overnight. Precipitate is filtered, dissolved in ethanol and cooled overnight.
Biswas et. al. (2015) used the flowering buds, the dried extract was absorbed on silica gel and
chromatographed over silica gel column packed in petroleum ether. The column was eluted
with chloroform : acetone : formic acid (8: 1.5 : 0.5), which gave yellow amorphous powder.
Salomon et. al. (2014) used the leaves in Microwave Assisted Extraction (MAE). Under
ranges of the variables such as time, power, and solvent of the microwave apparatus
mangiferin can be extracted. 10g of leaves powder was put into extraction vessel and was
refluxed with 200mL of water. And then filtered and subjected to identification tests.
Mango
Summary
Studies concerning antimicrobial property of Mangifera indica usually involves the extract of
leaves, fruit, and seed. Anti-microbial assays of those studies includes: disc diffusion method,
paper disk diffusion and agar well diffusion method.
RESULTS:
Sarabia et. al (2011) – Antibacterial Activity of Mangifera Indica (Carabao Mango) Fruit and Seed Extract
Biswas (2015) concluded that the solution of the isolated mangiferin from the flowering buds was found to
exert promising antibacterial activity against gram-positive (Staphylococcus aureus) and gram-negative
(Salmonella typhi) . The study found a very promising and readily available source for treating infections
caused by bacteria.
References
MANGO
Bhuvaneswari, K. (2013). Isolation of Mangiferin From Leaves of Mangifera indica L. Var. Alphonso.
Asian Journal of Pharmaceutical and Clinical Research, Vol. 6.
Biswas, T., Sen, A., Roy, R., Maji, S., Maji, H.S.(2015). Isolation of Mangiferin from Flowering Buds
of Mangifera indica L. And its Evaluation of in vitro Antibacterial Activity. Research & Reviews:
Journal of Pharmaceutical Analysis.
Sarabia, A.R., Acanto, C.M., Elizalde, C., Merosa, E.M. (2012). Antimicrobial Activity of Mangifera
indica (Carabao Mango) Fruit and Seed Extract. Medical Laboratory Science and Nursing.
University of Immaculate Conception.
De, P., & Pal, A. (2014). Effects of aqueous young leaves extract of Mangifera indica on gm (-)
microorganisms causing gastro-intestinal disorders. Asian Journal of Plant Science and
Research, 2014, 4(1):23-27
Salomon, S., Sevilla, I., Betancourt, R., Romero, A., Nuevas-Paz., Esquijarosa, A. (2014). Extraction
of mangiferin from Mangifera indica L. Leaves using microwave-assisted technique. Emir. J.
Food Agric. 2014. 26 (7):616-622. doi: 10.9755/ejfa.v26i7.18188.
United Nations. (2016). Mango: An INFOCOMM Commodity Profile. United Nations Conference on
Trade and Development.