COLUMN

CHROMATOGRAPHY
Created by :
 Bq. Ike Nursofia
 Dwi Ariani
 Laili Azmiati Arni
Ro’yal Aini
Rosika Armiyanti Maharani
Wardian Antoni

4/19/2019 Column Chromatography 1

Purification of Cinnamaldehyde
from Cinnamon Species by
Column Chromatography
by : Satya Nandam Sree, India
 Introduction

• Cinnamon sp : Family Laurance ( tropis and sub-

tropis area)
• Application : Herbal Medicine(antioxidant
activity)
• The Contents : cinnamaldehyde, eugenol,
cinnamic acid etc.

4/19/2019 Column Chromatography 3

 Sample Preparation
Bark of cinnamon was collected from the local
market at Visakhapatnam, AP. The bark was
cleaned and dried under sunlight for 24 h. The
dried bark was powdered. Cinnamon powder
was sieved by using different particle sizes
ranging from 354 to 125 microns.

4/19/2019 Column Chromatography 4

 Preparation the Extract
Sample + Filtrated
Erlenmeyer
methanol (whatman
set pH 5
50%(v/v) No.1)

made up to
+ n-hexane 50 mL with heated
distilledwater

Incubated ( 2
Result
H)

4/19/2019 Column Chromatography 5

 Column Chromatography
• Stationary phase : silica gel
• Mobile phase : methanol and n-
hexene

4/19/2019 Column Chromatography 6

 Packing column (dry packing )

Cotton sand Silica gel

Run the
mobile Sand
phase

4/19/2019 Column Chromatography 7

 Purification Mechanism

Add silica gel to

sample

Pour the sample

into column

Add mobile phase

continously

4/19/2019 8
Column Chromatography
 Result and Disccusion
• The best mobile phase is methanol
• The consentration of cinnamaldehyde that
observed to 44,6 mg/L and the consentration
was increased to 52 mg/L from the column
chromatography
• The purity of cinnamaldehyde was improved by
column chromatography

Column Chromatography
4/19/2019 9
4/19/2019 copyright 2006 10
www.brainybetty.com
4/19/2019 copyright 2006 11
www.brainybetty.com
4/19/2019 copyright 2006 12
www.brainybetty.com
4/19/2019 copyright 2006 13
www.brainybetty.com
 Conclusion
• The Contents of cinnamon sp are
cinnamaldehyde, eugenol, cinnamic acid etc
• The best mobile phase is methanol
• The consentration of cinnamaldehyde that
observed to 44,6 mg/L and the consentration
was increased to 52 mg/L from the column
chromatography
• The purity of cinnamaldehyde was improved by
column chromatography

4/19/2019 Column Chromatography 14