H2S producing organisms

• may produce so much

black precipitate (Ferrous
sulfide) that the acidity
of the butt is completely
masked. However, if it is
produced, an acid
condition does exist in
the butt, even not
observable should be
recorded as such
Gas production
• indicated by:
splitting of the
medium
a single gas bubble
complete
displacement of the
medium from the
bottom of the tube