Professional Documents
Culture Documents
By
Zaheer uddin
(Pharm-D,M Phil (pharmacy
practice) MBA( MARKETING)
DEFINATION:
• Biochemistry is the application of chemistry to the
study of biological processes at the cellular and
molecular level.
• It emerged as a distinct discipline around the
beginning of the 20th century when scientists
combined chemistry, physiology and biology to
investigate the chemistry of living systems by:
A. Studying the structure and behavior of the complex
molecules found in biological material and
Plasma Membrane
A lipid/protein/carbohydrate complex, providing a barrier and
containing transport and signaling systems.
Nucleus
Double membrane surrounding the chromosomes and the nucleolus.
Pores allow specific communication with the cytoplasm. The
nucleolus is a site for synthesis of RNA making up the ribosome
Mitochondrion
Surrounded by a double membrane with a series of folds
called cristae. Functions in energy production through metabolism.
Contains its own DNA, and is believed to have originated as a
captured bacterium.
Chloroplasts (plastids)
Surrounded by a double membrane, containing stacked thylakoid
membranes. Responsible for photosynthesis, the trapping of light
energy for the synthesis of sugars. Contains DNA, and like
mitochondria is believed to have originated as a captured
bacterium.
.
Golgi apparatus
A series of stacked membranes. Vesicles (small membrane
surrounded bags) carry materials from the RER to the Golgi
apparatus. Vesicles move between the stacks while the proteins are
"processed" to a mature form. Vesicles then carry newly formed
membrane and secreted proteins to their final destinations including
secretion or membrane localization.
Lysosymes
A membrane bound organelle that is responsible for degrading
proteins and membranes in the cell, and also helps degrade materials
ingested by the cell.
Vacuoles
Membrane surrounded "bags" that contain water and storage
materials in plants.
Peroxisomes or Microbodies
Produce and degrade hydrogen peroxide, a toxic
compound that can be produced during metabolism.
Cell wall
Plants have a rigid cell wall in addition to their cell membranes
Cytoplasm
enclosed by the plasma membrane, liquid portion called cytosol
and it houses the membranous organelles.
Cytoskeleton
Arrays of protein filaments in the cytosol. Gives the cell its
shape and provides basis for movement.
E.g. microtubules and microfilaments.
Plasma Membrane
• Boundary that separates the living cell from it’s
non-living surroundings.
• Provide shape
• Phospholipid bilayer
• Amphipathic - having both:
hydrophilic heads
hydrophobic tails
Phospholipid
• ~8 nm thick
• Is a dynamic structure
Membrane Functions
1. boundaries
6. Cell-cell
2. Localize adhesion
specific
functions
5. Cell-cell
communication
3. transport
4. Signal detection
Fluid Mosaic Model
Proteins embedded and floating in a sea of
phospholipids
Hydrophobic region
of protein
PROTEINS CAN
MOVE IN THE
MEMBRANE,
TOO!
Channel protein
Carrier protein
Cell recognition protein
Receptor protein
Enzymatic protein
Example of
Enzymatic
activity
associated
with a
membrane:
G-protein
cascade
Permeability of the Cell Membrane-
Differentially Permeable
Permeability of the Cell Membrane
DIFFUSION
• Diffusion
– the passive movement of molecules from a
higher to a lower concentration until
equilibrium is reached.
– How can we explain diffusion?
– Gases move through plasma membranes by
diffusion.
• Osmosis– A special case of diffusion
Process of diffusion
Gas exchange in lungs by diffusion
Osmosis
The diffusion of water across a
differentially permeable
membrane due to concentration
differences
Question:
What’s in a Solution?
Answer:
Which
way
will 3% NaCl solution
the 97% H2O 5% NaCl
water 95% H2O
move? Red Blood Cell
Hypotonic
Which
way
will 3% Na solution
the 97% H2O
1% Na
water 99% H2O
move? Red Blood Cell
Isotonic
• A solution with an equal solute concentration
compared to another solution.
Which
way
will 3% Na solution
the 97% H2O 3% Na
water 97% H2O
move? Red Blood Cell
ISOTONIC SOLUTION
HYPOTONIC SOLUTION
HYPERTONIC SOLUTION
Carrier Proteins
• Function—Transport. Are specific,
combine with only a certain type of
molecule.
• Types
–Facilitated transport--passive
–Active transport—requires energy
Facilitated Transport
Active Transport
The sodium-potassium pump
Exocytosis and Endocytosis
• Exocytosis---Cellular secretion
• Endocytosis—
–Phagocytosis— “Cell eating”
–Pinocytosis– “Cell drinking”
–Receptor-mediated endocytosis-
specific particles, recognition.
Exocytosis
Phagocytosis
Phagocytosis 1
Phagocytosis 2
Pinocytosis
Receptor-mediated Endocytosis
Receptors and signal molecules
• Cell-to-cell communication is essential for
multicellular organisms
• Biologists have discovered some universal
mechanisms of cellular regulation
Chemical signaling
between cells is one of the
most important ways that
activities of tissues and
organs are coordinated.
The nervous system is the
other major coordinating
system in animals, but even
here chemical signaling is
used between adjacent
neurons.
Modes of cell-cell signaling
1. Direct cell-cell or cell-matrix
2. Indirect: Secreted molecules.
signaling. The signaling molecules are hormones secreted by
endocrine cells and carried through the circulation system to act on
target cells at distant body sites.
signaling. The signaling molecules released by one cell act on
neighboring target cells (neurotransmitters).
Segment that
interacts with
G proteins
G-protein-linked receptor
• 2- Receptor tyrosine kinases are membrane
receptors that attach phosphates to tyrosines
• A receptor tyrosine kinase can trigger multiple
signal transduction pathways at once
Signal Signal-binding site
molecule
a Helix in the Signal
membrane molecule
Tyr Tyr
Tyrosines Tyr Tyr Tyr Tyr
Tyr Tyr
Tyr Tyr Tyr Tyr
Tyr Tyr
Tyr Tyr Tyr Tyr
Receptor tyrosine
kinase proteins Dimer
CYTOPLASM (inactive monomers)
Activated relay
proteins
Cellular
Tyr Tyr P Tyr Tyr P P Tyr Tyr P response 1
Tyr Tyr P Tyr Tyr P Tyr Tyr P
P
Tyr Tyr P Tyr Tyr P P Tyr Tyr P Cellular
6 ATP 6 ADP response 2
Plasma
Ligand-gated
membrane
ion channel receptor
Gate open
Cellular
response
Gate closed
The Specificity of Cell Signaling
• Different kinds of cells have different collections
of proteins
• These differences in proteins give each kind of
cell specificity in detecting and responding to
signals
• The response of a cell to a signal depends on
the cell’s particular collection of proteins
• Pathway branching and “cross-talk” further help
the cell coordinate incoming signals
Signal
molecule
Receptor
Relay
molecules
Activation
or inhibition
Response 4 Response 5