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10 µm
Figure 6.1
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• Concept 6.1: To study cells, biologists use
microscopes and the tools of biochemistry
Unaided eye
– Can be used to visualize different sized
cellular structures
10 m
Human height
1m
Light microscope
Length of some
nerve and
muscle cells
0.1 m
Chicken egg
1 cm
Frog egg
1 mm
Electron microscope
100 µm
Most plant
and Animal
cells
10 µ m
Nucleus
Electron microscope
Most bacteria
Mitochondrion
1µm
Proteins
Measurements
1 nm
Lipids 1 centimeter (cm) = 10−2 meter (m) = 0.4 inch
Small molecules 1 millimeter (mm) = 10–3 m
1 micrometer (µm) = 10–3 mm = 10–6 m
Figure 6.2 0.1 nm Atoms
1 nanometer (nm) = 10–3 mm = 10–9 m
50 µm
(b) Brightfield (stained specimen).
Staining with various dyes enhances
contrast, but most staining procedures
require that cells be fixed (preserved).
50 µm
Figure 6.5
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Homogenization
Tissue
cells
1000 g Homogenate
(1000 times the
force of gravity)
10 min Differential centrifugation
Supernatant poured
into next tube
20,000 g
20 min
80,000 g
Pellet rich in 60 min
nuclei and
cellular debris
150,000 g
3 hr
Pellet rich in
mitochondria
(and chloro-
plasts if cells
are from a Pellet rich in
plant) “microsomes”
(pieces of
plasma mem-
branes and Pellet rich in
cells’ internal ribosomes
membranes)
Figure 6.5
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
RESULTS In the original experiments, the researchers
used microscopy to identify the organelles in each pellet,
establishing a baseline for further experiments. In the next series of
experiments, researchers used biochemical methods to determine
the metabolic functions associated with each type of organelle.
Researchers currently use cell fractionation to isolate particular
organelles in order to study further details of their function.
Figure 6.5
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Concept 6.2: Eukaryotic cells have internal
membranes that compartmentalize their functions
• Two types of cells make up every organism
– Prokaryotic
– Eukaryotic
Figure 6.6 A, B
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• Eukaryotic cells
– Contain a true nucleus, bounded by a
membranous nuclear envelope
– Are generally quite a bit bigger than
prokaryotic cells
5
1
1
Total volume
(height × width × length 1 125 125
× number of boxes)
Surface-to-volume
ratio 6 12 6
(surface area ÷ volume)
Figure 6.7
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• The plasma membrane
– Functions as a selective barrier
Hydrophilic
region
Inside of cell
0.1 µm
Hydrophobic
region
(a) TEM of a plasma
membrane. The Hydrophilic
plasma membrane, region Phospholipid Proteins
here in a red blood (b) Structure of the plasma membrane
cell, appears as a
pair of dark bands
separated by a
Figure 6.8 A, B light band.
Nucleolus NUCLEUS
Rough ER Smooth ER
Chromatin
Flagelium
Plasma membrane
Centrosome
CYTOSKELETON
Microfilaments
Intermediate filaments
Microtubules Ribosomes
Microvilli
Golgi apparatus
Peroxisome
In animal cells but not plant cells:
Lysosomes
Lysosome Centrioles
Figure 6.9 Mitochondrion Flagella (in some plant sperm)
Central vacuole
Tonoplast
Golgi apparatus
Microfilaments
Intermediate
CYTOSKELETON
filaments
Microtubules
Mitochondrion
Peroxisome
Plasma membrane
Chloroplast
Cell wall
Plasmodesmata
In plant cells but not animal cells:
Wall of adjacent cell Chloroplasts
Central vacuole and tonoplast
Figure 6.9 Cell wall
Plasmodesmata
Nucleus
1 µm Nucleolus
Chromatin
Nuclear envelope:
Inner membrane
Outer membrane
Nuclear pore
Pore
complex
Rough ER
Surface of nuclear
envelope. Ribosome 1 µm
0.25 µm
Close-up of
nuclear
envelope
Ribosomes ER Cytosol
Endoplasmic reticulum (ER)
Free ribosomes
Bound ribosomes
Large
subunit
Small
0.5 µm subunit
TEM showing ER and ribosomes Diagram of a ribosome
Figure 6.11
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Concept 6.4: The endomembrane system
regulates protein traffic and performs metabolic
functions in the cell
• The endomembrane system
– Includes many different structures
Smooth ER
Rough ER Nuclear
envelope
ER lumen
Cisternae
Ribosomes Transitional ER
Transport vesicle
Smooth ER Rough ER 200 µm
Figure 6.12
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• There are two distinct regions of ER
– Smooth ER, which lacks ribosomes
– Metabolizes carbohydrates
– Stores calcium
– Detoxifies poison
Lysosome
Digestive
enzymes
Lysosome
Plasma membrane
Digestion
Food vacuole
Figure 6.14 A
(a) Phagocytosis: lysosome digesting food
Mitochondrion
fragment
Peroxisome
fragment
Lysosome
Digestion
Vesicle containing
damaged mitochondrion
Figure 6.14 B
(b) Autophagy: lysosome breaking down damaged organelle
• Contractile vacuoles
– Pump excess water out of protist cells
Central vacuole
Cytosol
Tonoplast
Nucleus Central
vacuole
Cell wall
Chloroplast
Figure 6.15 5 µm
Rough ER
Figure 6.16
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• Concept 6.5: Mitochondria and chloroplasts
change energy from one form to another
• Mitochondria
– Are the sites of cellular respiration
• Chloroplasts
– Found only in plants, are the sites of
photosynthesis
Intermembrane space
Outer
membrane
Free
ribosomes
in the
mitochondrial
matrix
Inner
membrane
Cristae
Matrix
Mitochondrial
DNA 100 µm
Figure 6.17
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Chloroplasts: Capture of Light Energy
• The chloroplast
– Is a specialized member of a family of closely
related plant organelles called plastids
– Contains chlorophyll
Chloroplast
Ribosomes
Stroma
Chloroplast
Inner and outer
DNA
membranes
Granum
1 µm
Figure 6.18 Thylakoid
Chloroplast
Peroxisome
Mitochondrion
Figure 6.19
1 µm
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Concept 6.6: The cytoskeleton is a network of
fibers that organizes structures and activities in
the cell
• The cytoskeleton
– Gives mechanical support to the cell
Table 6.1
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Microtubules
• Microtubules
– Shape the cell
Centrosome
Microtubule
Centrioles
0.25 µm
Figure 6.23 A
1 µm
Figure 6.23 B
15 µm
Central
microtubule
Outer doublets
cross-linking
proteins inside
Microtubules
Radial
Plasma spoke
membrane
Basal body
(b)
0.5 µm
0.1 µm
(a) Triplet
(c)
Dynein arm
(a) Powered by ATP, the dynein arms of one microtubule doublet
grip the adjacent doublet, push it up, release, and then grip again.
If the two microtubule doublets were not attached, they would slide
Figure 6.25 A relative to each other.
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Outer doublets ATP
cross-linking
proteins
Anchorage
in cell
(b) In a cilium or flagellum, two adjacent doublets cannot slide far because
they are physically restrained by proteins, so they bend. (Only two of
Figure 6.25 B the nine outer doublets in Figure 6.24b are shown here.)
Microvillus
Plasma membrane
Microfilaments (actin
filaments)
Intermediate filaments
Muscle cell
Actin filament
Myosin filament
Myosin arm
Parallel actin
filaments Cell wall
1 µm
Central vacuole
Cytosol
Plasma membrane
Plant cell walls
Core
protein
Fibronectin
Proteoglycan
Plasma molecule
membrane Integrins
Micro- CYTOPLASM
Integrin
filaments
Figure 6.29
– Adhesion
– Movement
– Regulation
Cell walls
Interior
of cell
Interior
of cell
– Desmosomes
– Gap junctions
TIGHT JUNCTIONS
At tight junctions, the membranes of
Tight junctions prevent Tight junction
neighboring cells are very tightly pressed
fluid from moving against each other, bound together by
across a layer of cells specific proteins (purple). Forming continu-
ous seals around the cells, tight junctions
prevent leakage of extracellular fluid across
A layer of epithelial cells.
0.5 µm
DESMOSOMES
Desmosomes (also called anchoring
Tight junctions junctions) function like rivets, fastening cells
Intermediate Together into strong sheets. Intermediate
filaments Filaments made of sturdy keratin proteins
Desmosome Anchor desmosomes in the cytoplasm.
Gap
1 µm
junctions GAP JUNCTIONS
Gap junctions (also called communicating
junctions) provide cytoplasmic channels from
one cell to an adjacent cell. Gap junctions
Extracellular consist of special membrane proteins that
Space matrix surround a pore through which ions, sugars,
between Plasma membranes Gap junction amino acids, and other small molecules may
cells pass. Gap junctions are necessary for commu-
of adjacent cells
nication between cells in many types of tissues,
Figure 6.31 0.1 µm including heart muscle and animal embryos.
Figure 6.32