Science Project

Recommendations (13)


http://www.chineseowl.idv.tw


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During our most recent weekly group meeting,
participants discussed efforts to elucidate the role that proteinase
inhibitors play in cancer cells. Commonly found in
legume seeds, proteinase inhibitors play a critical role in regulating
several physiological processes such as blood coagulation,
complement fixation, fibrinolysis, and fertilization. As proteins having
characteristic polypeptide scaffolds, most of these inhibitors are
grouped into several families including the Kunitz, Kazal, Burman Birk,
Serpin and mucus families. As well established, the production,
invasion and transfer of cancer cells are significantly related to
proteinase inhibitors. However, previous efforts have
failed to suppress cancer proteinase inhibitors effectively. (NOTE :
Add 3-4 sentences that describe characteristics of the problem or
statistics that reflect its severity) owing to the
inability to identify proteinase inhibitors that can suppress more than
80of all cancer cells. Consequently, the
inability to understand the physiological role of proteinase inhibitors in
plants makes it impossible to ultimately understand the role that
proteinase inhibitors play in cancer cells.
(:)
Therefore, we recommend purifying and
characterizing the coffee senna trypsin inhibitor (CSTI), to
which Coffee Senna, i.e. a leguminosae that belongs to its
bean sub-family, by using protein purification. Essential to
understanding the fundamental principles of protein
purification, protease inhibitors can be used to design new
substances, capable of effectively controlling diseases and
pathological processes. To do so, coffee
senna seeds can be analyzed. CSTI can then be extracted
using a crude extraction method containing ammonium
sulfate precipitate. Next, the senna trypsin inhibitor can then
be purified to utilize the column (i.e., gel filtration, affinity
chromatography, and ion exchange column) of various
characteristics (i.e., molecular size, molecular charge, as
well as molecular polar and affinity). Additionally, the purified
protein can be analyzed to further characterize its heat
stability and different SDS concentrations in order to achieve
stability.
(:)
As anticipated, analysis results can indicate that
CSTI can be extracted and the type of coffee senna seed
can be identified as well. Besides characterizing CSTI
precisely through analysis, its extraction and characterization
can be used to classify the coffee senna type. Moreover, the
CSTI sequence can achieve sequential alignment of the
protein with other legumes. In addition to
providing insight into the physiological significance of CSTI in
plants, results of this study can contribute to efforts to design
substances capable of effectively controlling diseases and
pathological processes. (NOTE : Add 2-4 more sentences
that describe more thoroughly how the proposed method
contributes to a particular field or sector)
(:)
At a recent meeting, members discussed the
enormous scientific potential role of Chinese herbal medicine,
especially in terms of industrial applications. Consumers
demand shampoos with natural ingredients and without any harmful
side effects. Manufacturers normally select natural plant additives and
attempt to reduce the chemical composition of such additives. This
combination often implies a relatively high retail price of shampoos.
Although traditional herbs from South Africa are frequently
used in shampoo, the use of China herbs in shampoo has received little
attention. Chinese herbs often depend on the use of fine trauma
medicines. Although non-toxic and mild, Chinese herbs are seldom
used in commodities such as shampoo. Certainly commercial
applications of Chinese herbal medicine still have many limitations, e.g.,
whether one ingredient has a particular effect, whether valid ingredients
can be extracted purely, or whether the usefulness of the ingredient is
depleted during the purification process, these problems. These issues
require further study. While Chinese herbal medicine has been
extensively studied for its oxidation ability to repress cancer cells, its
potential applications for external treatment such as in shampoos has
seldom been addressed.
(:)
In addition to numerous investigations,
Chinese herbal medicine can be found in many commercial
products. However, with the growing market in
pharmaceutical drugs and the health foods sector, Chinese
herbal medicine has many attractive features, e.g., anti-
oxidation capability, suppression of fungi, anti-cancer
capabilities, toxicity analysis, and analysis of particular
ingredients for research purposes.
Despite the extensive study of herbal medicine for
therapeutic treatment purposes that has yielded excellent
results with respect to antimicrobial activity of herbal plants,
Chinese herbal medicine has seldom been studied with
respect to its toxicity levels and potential industrial
applications.
(:)
Therefore, we recommend developing a novel
formulation involving the use of Chinese herbal medicine that
replaces that of the widely used South African herbal medicine.
To do so, Chinese herbal medicine can be
analyzed to suppress gram (-) bacteria. Cell culturing method is
then adopted to understand why Chinese herbal medicine does
not have negative side effects. Next, GC and HPLC methods can
be used to understand the adaptability of incorporating
compounds of Chinese medicine in shampoo. The proposed
formulation can enable five varieties of Chinese herbal medicine to
achieve a better outcome than South African herbal medicine can
in terms of suppressing gram (-) bacteria. As
anticipated, analysis results can indicate that , while traditional
South African herbs can restrain the microorganism effect with a
success rate of 90 upwards, Chinese herbs have the same
success rate of 99 upwards. This quality makes Chinese herbs
highly promising for efforts to restrain microorganisms and achieve
safe testing, implying safe production and substantial product
revenues.
(:)
The ability to suppress germs would enable use of flow
cytometer examination PGE2 to understand its anti-oxidation
activity, thus making Chinese herb medicine highly promising
for several commercial applications, including treatment for
athletes foot and the skin of head tinea owing to its ability to
effectively suppress fungi, as well as treatment of external
injuries owing to its ability as a disinfectant of germs.
In addition to providing a valuable reference for
Chinese herbal medicine research, the proposed formulation
can provide local healthcare product manufacturers with a
viable alternative to developing shampoos. Its desirable
features of effectively suppressing both fungi and fungi
owing to its anti-oxidation capability further increase its
commercial potential. (NOTE : Add 2 sentences that
describe more thoroughly how the proposed method
contributes to a particular field or sector)
Further details can be found at
http://www.chineseowl.idv.tw