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Proposal
Epidemiological study and molecular characterization of
Hydatid Cysts Isolated from human and some
Intermediate Hosts in Sulaimanya Province.
By
Abdullah Ahmed Hama
M.Sc. Biology/ Parasitology 2007
Supervisor
Prof. Wijdan Mero
Introduction
Hydatid disease is a silent zoonotic infection of
man and domestic animals caused by the larval
.stage of the small dog tapeworm
Man becomes infected accidentally by ingesting
the eggs of the parasite through hands, food, drink
.or other materials contaminated with dog feces
Introduction
Hydatid cyst after surgery
The disease is endemic or hyper endemic in
most of the Middle East countries mainly in
.communities where animal husbandry is common
Introduction
The identification of the strains responsible
for human echinococcosis is nowadays a
significant point which has to be taken into
consideration in order to focus and to adapt
the control measures and the means of
.diagnosis
: The life cycles
Life cycle involve two mammalian hosts .The adult cestode
inhabits the small intestine of a carnivore and produces eggs
containing infective oncospheres. Either cestode segments
.(proglottids) containing eggs or free
Life cycle
Location of the cyst
Aim of this study
: The objectives of the present study are to
Aim
Sample Collection
1
from animal Epidemiological analysis for 5
year for human and animals
Histological confirmation
Direct M.E sulaimania
kalar
Protoscolecies by centerfugation Germinal layer
halabja
penjween
DNA extraction Saeed sadq
cyst from liver lung, spleen and other organ by 10 ml Serum from
surgery Butcher
Shepherd
Germinal layer Aspiration of fluid
Grocer
separated
House wife
Hisological conformation D. M. Examination
animal husbandry
Abattoir worker
DNA extraction
Veterinarian
Control normal person
PCR amplification of CO1 gene and
using specific primer
ELISA IgG detection
Genetic diversity (genotyping) for human, goats,
sheep and cattle
Positive Negative
End