M a te o Budinich

BIO396: TOPICOS AVANZADOS EN BIOQUIMICA INDUSTRIAL

My background in 13 years

Innovative professional with a comprehensive education and progressive expertise in scientific research. Accomplished in leading and participating in product development teams. Effective in managing multiple and concurrent responsibilities with good communication skills. A thirst for employing new ideas, concepts, and technologies. Skilled in use of small scale to pilot plant size equipments, such as fermentors, evaporators, spray driers, mixers and membrane filters. Know-how of statistical experiment design, product development, product positioning applications via Principal component analysis, Response surface method, and mathematical modeling techniques. applied extensively in the area of nutraceuticalsand dairy microbiology. Knowledgeable in the use of qualitative and preparative chromatographic separations (GC, GC-MS, TLC, HPLC, LC-MS), spectroscopic methods (UV-Vis, FT-IR, NMR), Protein characterization (Size exclusion, Ion-exchange chromatography, 2D-Gel electrophoresis), Immunoassay and molecular biology. Competent in tissue culture and in vitro cell assays.

Your background? In 2 min

ABARZUA PASTENE SEBASTIAN ALEJANDRO ANDRADE AAZCO CLAUDIA ANDREA BRAVO LOPEZ ROSSMARY OLGA EJSMENTEWICZ VALERO TROY ALEJANDRO FIGUEROA FIGUEROA DANIELLA RUTH FREZ MUOZ LUCIA DE LOS ANGELES JELDES JEREZ EMANUEL MUJICA INAIMAN KAREN DENISSE NEUMANN GAYTAN GERMAN ENRIQUE PERALTA ESPINOZA CAMILA ANNABELLA PRIETO PINCHEIRA CRISTIAN JAVIER VALDERRAMA FONTECILLA IGNACIO ANDRES

Your expectations for this class are?

My expectations for this class are:

Continuous discussion in a favorable environment:
Respectful language All of us are colleagues!!

I prefer to be stopped than wait to the end of the topic ALL QUESTIONS ARE VALID!

T omamos un break?

10 minutos

Metodologia: Clases
Clases: Inicialmente los tres mdulos

sern dedicados a clases y una vez definido los temas de seminario, el tercer modulo ser dedicado a los seminarios Asistencia a los seminarios es obligatoria para todo el curso

Metodologia: Evaluacion
Solemnes. Estas sern de una hora de 45

preguntas de eleccin mltiple y/o respuestas cortas de UNA o DOS frases. Las solemnes sern durante los primeros 60 minutos de clase y luego de 15 minutos se continuarn con los seminarios. Examen: Constar de 4 preguntas de desarrollo en base a un caso de bioqumica industrial. Las cuatro preguntas estarn asociadas entre si. Cada pregunta deber ser contestada en un procesador de texto de no ms de una pgina con tipografa Times New Roman de 10 pts, espacio simple. Dispondrn de uso de cualquier material disponible para los alumnos siempre y cuando este sea debidamente citado.

Metodologia: Seminarios
El objetivo del seminario es para que el alumno

elija DOS temas que le interese en el rea de la bioqumica industrial Seminario I: Su duracin ser de 10 minutos ms 5 minutos de preguntas de la clase.
El alumno ser evaluado de forma objetiva por la

clase y el mismo, mediante el llenado de una encuesta de presentacin el profesor y el presentador

La participacin de seminarios ser evaluada por La presentacin en formato Microsoft Office

Powerpoint deber ser entregada va correo electrnico a mas tardar 24 horas antes del inicio de la clase para poder optar al puntaje mximo

Metodologia: Seminarios
Seminario II: ser de 20 minutos y 5

minutos de preguntas.

Tendr que ser acompaado de un

resumen escrito en Microsoft Office Word de una pgina con tipografa Times New Roman de 10 pts, espacio simple, el cual ser enviado al profesor por correo electrnico a mas tardar 24 horas antes de la clase.

Se recomienda enviar los documentos

48 horas antes para poder contar con la posibilidad de recibir comentarios y/o correcciones por parte del

Bibliografa Obligatoria
Suministrados por el profesor al

comienzo de cada unidad va email

Presentaciones y artculos cientficos

de cada unidad estarn disponibles va email 48 horas antes de la clase

Contacto:

mateo.budinich@yahoo.com

solo para situaciones de indole personal

BIO396-UNAB: Group home page:

http://groups.yahoo.com/group/BIO396

-UNAB

archivos del curso, clases, literatura de

apoyo, papers

Group email address:

BIO396-UNAB@yahoogroups.com Discusion sobre temas y dudas de las

clases

http://groups.yahoo.com/group/BIO396-UNAB

Archivos de la clase

Li ra tu ra su g e ri a , a rch i p re se n ta ci n te d vo o

Polls o encuestas

I p o rta n te p a ra e n te n d e r co m o va l cl se , a q u i ta m b i n se e va l a ra n l s se m a a e u o

Calendario

A q u i p o n d re e l ti l d e l cl se tu o a a C a m b i s a l d i n o rm a l d e cl se s, o a a e j se m i a ri s : n o

INTRODUCTION

Biochemical engineering Bioprocess Biotechnology Biotechnology application

Definition (one of many)

Biochemical engineering is concerned

with conducting biological processes on an industrial scale This area links biological sciences with chemical engineering The role of biochemical engineers has become more important in recent years due to the dramatic developments of biotechnology

Biological processes

Definitions Bioprocess design goals Bioprocess advantages and disadvantages Industrial Microbiology setup and flowchart

Biological processes
Industrial applications of biological

processes are to use living cells or their components to effect desired physical or chemical changes.
To carry out a bioprocess on a large

scale, biochemical engineers need to work together with biological scientists

Bioprocess design and development goals

Typical biological process

Bioprocess design and development goals

to obtain the best biological catalyst

(microorganism, animal cell, plant cell, or enzyme) for a desired process to create the best possible environment for the catalyst to perform by designing the bioreactor and operating it in the most efficient way to separate the desired products from the reaction mixture in the most economical way

Basic questions to consider when designing and developing a bioprocess

Bioprocess design and development:

What change can be expected to occur?

understanding of the .basic sciences

for the process involved the biological catalyst may be selected or genetically modified with a consideration of the largescale operation.

Bioprocess design and development:

How fast will the process take place?

Kinetics deals with rate of a reaction and how it

is affected by various chemical and physical conditions.

Similar techniques can be employed to deal

with enzyme or cell kinetics.

It is important to know how the rate of the

reaction is influenced by various operating conditions. transport phenomena, biological interactions, clonal stability, and so on.

This involves the study of thermodynamics,

Bioprocess design and development:

How can the system be operated and controlled for the maximum yield?
reliable on-line sensing devices need to be

developed.

on-line optimization algorithms need to be

developed and used to enhance the operability of bioprocess and to ensure that these processes are operated at the most economical points

Bioprocess design and development:

How can the products be separated with maximum purity and minimum costs?
standard separation techniques developed in

chemical processes such as distillation, absorption, extraction, adsorption, drying, filtration, precipitation, and leaching.

many novel techniques have been developed

to separate or to analyze biological materials on a small laboratory scale, such as chromatography, electrophoresis, and dialysis.

Bioprocess design and development

Advantages and Disadvantages

Bioprocess design and development:

Advantages
Mild reaction condition
the typical condition is at room

temperature, atmospheric pressure, and fairly neutral medium pH.

the operation is less hazardous, and the

manufacturing facilities are less complex compared to typical chemical processes.

Bioprocess design and development:

Advantages
Specificity
an enzyme catalyst is highly specific and

catalyzes only one or a small number of chemical reactions.

a great variety of enzymes exist that can

catalyze a very wide range of reactions

Bioprocess design and development:

Advantages
Effectiveness
the rate of an enzyme-catalyzed reaction

is usually much faster than that of the same reaction when directed by nonbiological catalysts.
a small amount of enzyme is required to
produce the desired effect.

Bioprocess design and development:

Advantages
Renewable resources
the major raw material for bioprocesses is

biomass
provides both the carbon skeletons and

the energy required for synthesis for organic chemical manufacture

Bioprocess design and development:

Advantages
Recombinant DNA technology
the development of the recombinant DNA

technology promises enormous possibilities

to improve biological processes

Bioprocess design and development:

Disadvantages
Complex product mixtures
multiple enzyme reactions are occurring in

sequence or in parallel,
the final product mixture contains cell

mass, many metabolic by-products, and a remnant of the original nutrients. components.

the cell mass also contains various cell

Bioprocess design and development:

Disadvantages
Dilute aqueous environments
the components of commercial interests

are only produced in small amounts in an aqueous medium.

separation is very expensive. traditional separation techniques cannot

be employed.

novel separation techniques that have

been developed for analytical purposes, need to be scaled up

Bioprocess design and development:

Disadvantages
Contamination
easily contaminated, since many

environmental bacteria and molds grow well in most media.

the problem becomes more difficult with

the cultivation of plant or animal cells

Bioprocess design and development:

Disadvantages
Variability
cells tend to mutate due to the changing

environment and may lose some characteristics vital for the success of process.
enzymes are comparatively sensitive or

unstable molecules and require care in their use.

Typical setup in an Industrial Microbiology Establishment

Flowchart of the Production Process

Biotechnology

Definitions Interdisciplinary nature

Some selected definitions of biotechnology

A collective noun for the application of biological

organisms, systems or processes to manufacturing and service industries.

The integrated use of biochemistry, microbiology and

engineering sciences in order to achieve technological (industrial) application capabilities of microorganisms, cultured tissue cells and parts thereof.

Some selected definitions of biotechnology

A technology using biological phenomena for copying

and manufacturing various kinds of useful substances.

The application of scientific and engineering

principles to the processing of materials by biological agents to provide goods and services.

Some selected definitions of biotechnology (continued)

The science of the production processes based on the

action of microorganisms and their active components and of production processes involving the use of cells and tissues from higher organisms. Medical technology, agriculture and traditional crop breeding are not generally regarded as biotechnology.

Some selected definitions of biotechnology (continued)

Really no more than a name given to a set of

techniques and processes.

The use of living organisms and their components in

agriculture, food and other industrial processes.

The deciphering and use of biological knowledge. The application of our knowledge and understanding

of biology to meet practical needs.

The interdisciplinary nature of biotechnology

T omamos un break?

10 minutos

Biotechnology applications:

Pharmaceuticals

Biotechnology applications: Pharmaceuticals

antibiotics, antigens (stimulate antibody response), endorphin (neurotransmitter), gamma globulin (prevent infections), human growth hormone (treat children with dwarfism), human serum albumin (treat physical trauma), immune regulators, insulin, interferon (treat infection), interleukins (treat infectious decease or cancer), Iymphokines (modulate immune reaction), monoclonal antibody (diagnostics or drug delivery), neuroactive peptides (mimic the body's pain-controlling peptides), tissue plasminogen activator (dissolve blood clots), vaccines

Biotechnology applications:

Animal Agriculture

Biotechnology applications: Animal Agriculture

development of disease-free seed

stocks, healthier food animals, higher-yielding food animals

Biotechnology applications:

Plant Agriculture

Biotechnology applications: Plant Agriculture

transfer of stress-, herbicide-, or pest-

resistance traits to crop species, development of plants with the increased abilities of photosynthesis or nitrogen fixation, development of biological insecticides and non-ice nucleating bacterium.

Biotechnology applications:

Specialty Chemicals

Biotechnology applications: Specialty Chemicals

amino acids, enzymes, vitamins, lipids, hydroxylated aromatics, biopolymers

Biotechnology applications:
E n vi n m e n ta l ro A p p l ca ti n s i o

Biotechnology applications: Environmental Applications

mineral leaching, metal concentration, pollution control, toxic waste degradation, enhanced oil recovery.

Biotechnology applications:

C om m odi C hem i l ty ca s

Biotechnology applications: Commodity Chemicals

acetic acid, acetone, butanol, ethanol, many other products from biomass conversion processes.

Biotechnology applications:

Bioelectronics

Biotechnology applications: Bioelectronics

biosensors, biochips

http://www.oycus.com/en/market-segments/industrial-biochemistry .html

Clinical chemistry reagents

Immunochemistry reagents

Biocatalyst

Biotechnology tree
YOU HAVE THE

BASICS! We will cover the tree during this semester THE REST IS FOR YOU TO GRAB!!!