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Culture Documents
Jaime Pascual
Innate immunity:
first line of defense against microbes
Three arms for innate immune detection
of pathogens
Toll-like receptor signaling pathway
κ
Suzuki et al, Trends in Immunology, 2002
Crystal structure of human Toll-like
receptor 2 TIR domain
(1) TIR-like gene; (20) integrase gene; (12) type IV secretion pilin protein precursor gene
Phylogenetic M.acetivorans NP 617753.1-20091678 1-95/
distribution
M.mazei NP 632249.1-21226327 1-95/186
T.erythraeum Tery p 3677-23043038 111-21
T.elongatus tlr1460-22299003 335-441/860
N.punct. p 2340-23126020 6-112/523
B.abortus RBV02711
B.melitensis 114-217/250 114-217/2
BMEI1674-17987957
E.coli CFT073
S.enteritidis NP 754290.1-26248250
RSEN05207 102-206/228166-2
C.aurantiacus RCAU04526
S.aureus RSAY02557 2-91/91
138-242/280
C.tepidum CT1466-21674286 1-101/315
A.tumefaciens ymc-10955119
N.punct. p 3951-23127638 234-337/371
1-99/319
S.coeli. SC5G9.14-21218854 16-126/959N.punct. p 2323-23126003 2-102/308
S.coeli. SC7H1.23-21224291 4-112/913 S.sp. slr0658-16332137 2-107/331
S.coeli. StBAC16H6.31-21224333 30-137/93 R.solana. RSp0130-17548351 366-468/521
C.crescentus CC1413-16125662 3-124/688 M.loti msi144-2080397238-145/178
H.mobilis RHMO01074 2-117/813 B.suis BR0735-23501622 137-244/277
F.nucleatum ATCC 25586-19704057
Phyla:
B.melitensis BMEI1216-1798749938-145/178
G.metallireducens Gmet p 2197-23055336 9 C.thermocellum Chte p 1504-23021423 1-96
N.punct. p 809-23124445 283-387/412 B.fungorum Bcep p 4892-22986961 1-95/431
R.sp. y4lF-16519836 1-103/323 N.punct. NP 485998.1-17229450 232-329/10
X.axonopodis XAC3296-21244022 1-105/340 M.degradans Mdeg p 785-23026951 7-104/37
Euryarchaeota R.palustris RRPA00954 1-107/320
N.punct. p 232-23123829 1-106/247
Anabaena.sp RAN00789 1-102/363
N.punct. p 1378-23125040 1-97/371
N.punct. p 233-23123830 1-105/532 R.albus TIGR 207-308/348
N.punct. p 4631-23128321 1036-1144/1185
Proteobacteria N.punct. p 4808-23128502 960-1068/110
Y.pestis y2426-22126310 132-229/358
G.metallireducens Gmet p 2193-23055332 1
P.gingivalis RPG00640 1064-1166/1232 T.fusca Tfus p 2407-23019318 1-109/255
M.barkeri RMBA03625 195-295/969 H.sapiens SARM-14149667 523-629/690
N.punct. NP 485998.1-17229450 434-531/10 M.musculus
Firmicutes LOC195039-25056757 101-207/26
N.punct. NP 485998.1-17229450 1-99/1047 C.elegans 3E960-17553056 703-809/930
S.coeli. SC6A9.25-21223993 8-123/254 D.melanogaster CG7915-24660603 808-914/1
R.sp. y4cD-16519676 182-285/640 A.gambiae agCG54245-21298926 605-711/744
E.faecium REFA01101 73-174/283 S.coeli. SC6A9.38-21223984 1-99/886
Cyanobacteria H.pylori jhp1330-15612395
H.pylori
N.punct.HP1437-15646046
R.rubrum
p 4079-23127767
L.mesenteroides
74-161/187
Lmes
S.coeli. SCD82.03-21223014 1-99/966
81-181/239
S.coeli. RSX14993
25-133/425
S.coeli.
00014575.1-22966980
E.faecalis
1-95/191
SCC88.13c-21221060
REF01505
pRMN02248
189-23023477 1-103/274
124-
563-664/705
M.barkeri ZP
S.hominis B.subtilis
G.strain yddK-16077567
00078625.1-23051929
C.thermocellum
M.magneto.
S.mutans
RCTH02496
RMMA03087
BAB83481-18148891
RGEM10173 1-111/324
4-83/83 109-209/266
1-98/10
1-103/158
2-116/490
1-108/306
M.haemolytica RPQ02288 3-101/101
Chlorobi
Chloroflexi
Actinobacteria
Planctomycetes
Fusobacteria
Bacteroidetes
Metazoa
aims
• 3D structure of a prokaryote TIR domain
– Comparison with a eukaryote TIR receptor
Full length
C-terminal
fragment
(S146-A-M-K-P)
Gel filtration chromatograms for PdTLP
aa1 Leu145 aa299
Coiledcoil TIRlike
pI 10.2 Full length pI 9.6 pI 5.7
600
300
mAU Full length
200
(33 kDa)
100
0
0 20 40 60 80 100
ml
-10
-20 Oligomer
-30 N-terminal
Coiled-coil
-40
mAU
-50
-60
-70
(16 kDa)
-80
0 20 40 60 80 100
ml
600
500
Monomer
400
300
C-terminal
mAU
200 TIR-like
100
(17 kDa)
0
-100
0 20 40 60 80 100
ml
Differential scanning calorimetry profiles for
PdTLP full length, coiled-coil & TIR-like domains
Circular dichroism spectrum of
the coiled-coil region of PdTLP
H-15N 2D HSQC NMR spectrum of PdTLP
1
TIR-like domain
(ppm)
(ppm)
Prokaryote TIR-like proteins suppress
NFkB activation induced by MyD88
Ray Low
Robert C. Liddington
Genaro Pimienta
John C. Reed
Takashi Mukasa
Adam Godzik
“Second life” avatar:
jamie pascal