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W cos|s |s te anaerob|c
catabo|s of gcose t doesnt
req|re oxgen so |s descr|bed as
anaerob|c resp|rat|on (w|tot
a|r) @erefore |t does not |nvove
an react|on |n w|c C2 |s
absorbed or Co2 |s reeased
W cos|s as a net |ed of two A@9
@s gcos|s |s a patwa of ten
react|ons |n w|c one s|xcarbon
oece of gcose |s sp|t (weter
or not oxgen |s present |nto two
|dent|ca treecarbon oeces
eac caed prv|c or prvate
W @e process |nvoves a ser|es of
enze react|ons tat
sccess|ve break sgars sc as
gcose gcose 19 or frctose
set down |nto 3carbon oece
caed prv|c ac|d |n te
ctopas ots|de an organee
1. Phosphorylation of glucose to glucose-6-
phosphate
2. Isomerisation of glucose-6-phosphate into
fructose-6-phosphate
3. Fructose-1,6-bisphosphate is broken down to
glyceraldehyde-3-phosphate and dihydroxy-
acetone phosphate by aldolase
4. 4. Dihydroxyacetone Dihydroxyacetone phosphate is phosphate is isomerized isomerized to to
glyceraldehyde glyceraldehyde- -33- -phospha phospha te te by the enzyme by the enzyme
triose triose phosphate phosphate isomerase isomerase
5. 5.
|rst Stage of cos|s
$econd $tage of Glycolysis $econd $tage of Glycolysis
11- -Glyceraldehyde Glyceraldehyde- -33- -phosphate is converted to phosphate is converted to
1,3 1,3- -bisphosphoglycerate by the enzyme bisphosphoglycerate by the enzyme
glyceraldehyde glyceraldehyde- -33- -dehydrogenase dehydrogenase
22- -1,3 1,3- -bisphosphoglycerate is converted to 3 bisphosphoglycerate is converted to 3- -
phosphoglycerate by phosphoglycerate by phosphoglycerate phosphoglycerate
kinase kinase
33 pospogcerate |s converted to 2 pospogcerate |s converted to 2
pospogcerate b te enze pospogcerate b te enze pospogcerate pospogcerate
tase tase
88 22 pospogcerate |s converted to pospogcerate |s converted to
pospoenoprvate pospoenoprvate (99) b te enze (99) b te enze enoase enoase
99 9ospoenoprvate 9ospoenoprvate |s converted to |s converted to prvate prvate b b
te enze te enze prvate prvate k|nase k|nase
Steps of
gcos|s |n
deta|s
19osporat|on of gcose
W cose pospate |s te start|ng
ater|a n a t|sses te
posporat|on of gcose |s
catazed b exok|nase
W cose + A@9 cose
6pospate + AD9
2soer|zat|on of gcose6
pospate
W @e |soer|sat|on of gcose6
pospate to frctose6p |s
catazed b pospogcose
|soerase @e react|on |s read|
revers|be
W cose6pospate
rctose rctose 66 pospate pospate
3posporat|on of frctose
6pospate
W @e |rrevers|be posporat|on
react|on catazed b pospo
rctok|nase
rctose 16 rctose 16 8|s 8|s p + AD9 p + AD9
% %rctose 6 pospate + A@9 rctose 6 pospate + A@9
Cearvage of frctose 16
b|spospate
W Adoase |s a enze w|c ceaves frctose
16 b|sp to d|droxacetone pospate
and gceradedes 3pospate @e
react|on |s revers|be and |s not sb[ect to
regat|on
rctose 16 rctose 16
b|opospate b|opospate
ceradede ceradede 3 p 3 p
D|droxacetone D|droxacetone 3p 3p
soer|zat|on of d|drox acetone
pospate
W @r|ose pospate |soerases catazes
te revers|be |nterconvers|on of
d|droxacetone3pospate to
gceradede 3 p becase
D|droxacetone pospate cannot
proceed frter on te d|rect patwa
of gcos|s
W and st be |soer|zed to
gceradede 3p for frter
etabo|s |n te gcos|s seqence
@|s |soer|zat|on rests |n te
prodct|on of two oeces of
gceradedes pospate fro te
ceavage prodcts of frctose 16
b|spospate
W D|drox acetone
ceradede3p
6Cx|dat|on of gceradede 3
pospate
W @e ox|dat|on of te Adede grop
of gceradede3 pospate to a
carbox grop |s coped to te
attacent of 9| to te carbox
grop @e |g energ pospate
grop at C
1
of 13
d|pospogcerate
Conserves much of the free energy
produced by the oxidation of
gIyceraIdehyde-3 phosphate
W 2ceradede3p + 2NAD+9
2 13 8| pospogcerate +2 NADn + n+
orat|on of A@9 fro 13
d|pospogcerate and AD9
W @e |generg pospate grop of 13
8|pospogcerate |s sed to sntes|ze A@9
fro AD9 |n a react|on catazed b
pospogcerate k|nase
W 13 8|pospogcerate + 2 AD9
W 3pospogcerate + 2 A@9
8S|ft of te pospate grop fro C3 to
C2
W @e s|ft of te pospate grop
fro carbon #3 to carbon #2
catazed b pospogcerotase
|s free revers|be
W 3b|pospogcerate
22 pospogcerate pospogcerate
9Dedrat|on of 2pospogcerate
W @e dedrat|on of 2pospogcerate
catazed b enoase red|str|btes te
energ w|t te 2pospogcerate
oece rest|ng |n te forat|on of
pospoenoprvate (99)
W w|c conta|ns a |generg eno
pospate @e react|on |s revers|be
desp|te te |generg natre of
te prodct
W 2pospogcerate
pospoeno pospoeno prvate prvate
10orat|on of prvate
W @e eq||br| of te react|on
catazed b prvate k|nase |s far to te
r|gt favor|ng te forat|on of A@9
W pospoeno prvate+AD9
9rvate 9rvate + A@9 + A@9
portant fnct|on of gcos|s
@e forat|on of oeces tat
can be reoved fro te
patwa to sntes|ze severa
oter const|tents of w|c te
pant |s coposed
9rodct|on of soe A@9 fro
gcos|s patwa
prodct|on of anoter oece
NADn tat we a conven|ent
cons|der to energ r|c @e energ |n
NADn a be sed |n e|ter of two
was
W |rst NADn a enter te |tocondr|a
were eectron transport react|ons
ox|d|ze |t and convert te energ |nto
te ter|na pospate bond energ of
two A@9s f t|s appens te two NADn
oeces ar|s|ng fro eac exose a
be cons|dered eqa to te read|
ava|abe energ of for A@9 oeces
W Second NADn (or NAD9n) can
be sed as a sorce of eectrons
|n neros anabo|c react|ons
Note that the addition of a phosphate to an Note that the addition of a phosphate to an
ADP to form ATP is called phosphorylation. ADP to form ATP is called phosphorylation.
For every glucose molecule (6C), the net For every glucose molecule (6C), the net
result is the formation of 2 ATP and 2 NADH result is the formation of 2 ATP and 2 NADH
molecules molecules
Input Input Output Output
Glucose Glucose 2 2 pyruvate pyruvate
2 2 NAD NAD
++
2 2 NADH NADH
2 2 ADP ADP 2 2 ATP ATP
Net result of glycolysis
ate of prvate to Co2
Acoo ferentat|on
W n ost pant ces etano and Co2 are
te a|n prodcts of anaerob|c
etabo|s w|t saer aonts of
act|c ac|d fored n acoo
ferentat|on prv|c ac|d |s converted
|nto et acoo and carbon d|ox|de
w|t acetadede f|gr|ng as
|ntered|ate
W t |nvoves two steps|n f|rst step |n te
presence of enze prv|c decarboxase
te ac|d grop |s reoved b
decarboxat|on and prv|c ac|d |s
converted to
9rv|c 9rv|c ac|d ac|d + + acetadede acetadede + + Co Co
22
acetadede acetadede + + Co Co
22
W n te second step |n te presence of
acoo dedrogenase and NADn
2
acetadede |s redced to et
acoo
W Cn
3
Cno + NADn
2
Cn3 Cn3 Cn2 Cn2 on + NAD on + NAD
. The only goal oI Iermentation reactions is . The only goal oI Iermentation reactions is
to convert NADH to NAD to convert NADH to NAD