Professional Documents
Culture Documents
Check results from week 2. Record results in templates. Organisms studied week 2
Culture characteristics
Use supplement and handout to observe the growth of the four organisms in the slant, deep, broth, and on the plate. Do the organisms look like one of the examples on your sheet? Try to record their appearance on your templates
Serratia
Colony morphology
Colony morphology
Elevation
Slants
Is there growth in the bottom ? Is there growth on the slant itself What are the growth characteristics on the slant? Key words Aerobic Anaerobic Facultative
When you have finished observing all of your cultures Place all tubes in rack in hazardous waste Place all plates in cans in hazardous waste Wipe down desk top
Preparation
Label all tubes and plates carefully Assign each member of the group 2 organisms Transfer the organisms to the culture media using aseptic techniques used in weeks one and two
Gram negative organisms PA - Pseudomonas aeruginosa PV- Proteus vulgaris EC- Escherichia coli EA- Enterobacter aerogenes Gram Positive Organisms BS - Bacillus subtilis SA - Staphylococcus aureus SE - Staphylococcus epidermidis
SS- Streptococcus salivarius
Aseptic techniques Culture transfer techniques Media Safety precautions Microscope identification