Professional Documents
Culture Documents
5 14 Biotechnology
Trial Materials
outline the principles of modern biotechnology and explain its significance now and for the future
Looking at Micro-organisms
Types & Size - Microscopy
Hanging drop preparation
Algae Protozoa Wet preparation Yeast Prepared slides/photographs Bacteria Viruses
Microbiology Techniques Microbiology techniques Cards Micro Safety and Techniques CD-Rom from SSERC Contact SSERC in 1st instance
Growing Micro-organisms
- effect of temperature
Inoculate plates with yeast
refrigerator room
room
room
oven
room
Growing Micro-organisms
- where are they found?
inoculate control test
Seal plates Incubate room Temp
control
test
Do not re-open
Autoclave
Harmful Micro-organisms
- bread mould investigation
Use different types of bread
White Brown Use double self-seal plastic bags
Organic
Non-organic Long-life
Do not open
Autoclave on completion of investigation
Harmful Micro-organisms
- effect of cleaning
unwashed water unwashed water
disinfectant
disinfectant
Harmful Micro-organisms
- antifungals
control
Helpful Micro-organisms
- dough race
1 Flour Water 2 Flour Yeast 3 Flour
Yeast
Sugar
Greater respiration in 3 causes greater CO2 production and dough to rise further
Keep materials cool where indicated Squash fruit in plastic bag to mechanically break up cells Add washing up liquid, salt and heat to 60C for 15 min to disrupt cellular membranes Filter to remove cell debris Precipitate by adding cold methanol
Practical introduces the idea of separating mixtures and using bands to assist in identification