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Culture Documents
Dr.T.V.Rao MD
DR.T.V.RAO MD
WHAT IS LISTERIOSIS
Listeriosis, a serious infection caused by eating food contaminated with the bacterium Listeria Monocytogenes, has recently been recognized as an important public health problem in the United States. The disease affects primarily persons of advanced age, pregnant women, new-borns, and adults with weakened immune systems. However, persons without these risk factors can also rarely be affected.
DR.T.V.RAO MD
Listeria Monocytogenes is a rod-shaped aerobic and gram positive pathogenic bacterium that invades the cytoplasm of living cells. It develops a distinctive rocket tail structure to help push through the cytoplasm. Eventually, these "rockets" push bacteria into neighboring cells, propagating the infection
DR.T.V.RAO MD
LISTERIOSIS
Listeriosis is caused by Bacterial agent an intracellular pathogen Listeria Monocytogenes L.monocytogenes produces infections world wide
DR.T.V.RAO MD
LISTERIA MONOCYTOGENES
L.monocytogenes is a Gram + ve non spore forming. A specific character of the organism manifest with tumbling end or over end motility at 220c 280c but not at 370c But makes the Microbiologists to identify from Diptheriods, which are mistaken and specimens are discarded.
DR.T.V.RAO MD
BIOCHEMICAL REACTIONS
Bacteria are facultative anaerobic microbes
Catalase + motile
Listeria produce acid and not gas in various sugar fermentation tests
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PATHOGENESIS AND PATHOLOGY Listeria Monocytogenes enters through the Gastro intestinal tract after infections of contaminated foods such as cheese or vegetables, The cell wall surface protein called Interanalin interacts with E CADHERIN and enters into epithelial cells Bacteria produce Listeriolysin L.monocygenes can move from cell to with out being exposed to Antibodies,Complement,Polymorphonuclear cells
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Lymphomas
Organ transplant recipients
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The bacterium has been found in uncooked meats and vegetables, soft cheeses, deli cold-cuts, and unpasteurized milk or foods. If acquired at birth, the incubation period is 7 to 28 days.
SYMPTOMS
Fever
Muscle ache
GI Sx: Nausea, diarrhea Pregnant women: mild flu-like Sx, miscarriage, still birth, premature delivery, or infected newborn.
If infection spreads to the nervous system: headache, stiff neck, confusion, loss of balance, or convulsions.
Lethargy
irritability
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Nausea;
Cramps; Diarrhea; Severe Headache; Constipation; or Persistent fever.
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LISTERIOSIS IN ADULTS
Adults may present with bacterimia . Meingoencephalitis and occur most commonly in Immunosupressed patients in whom Listeria is one of the more common cause of Meningitis Disease can be insidious to fulminant
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DIAGNOSIS
There is no routine screening test for susceptibility. Patient may present with fever, or stiff nec, . A blood or spinal fluid examination (to cultivate the bacteria) will confirm the clinical diagnosis. . During pregnancy, patients need detailed investigations to rule our Listeriosis.
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DIAGNOSIS
Diagnosis dependent on isolation of Organisms in cultures obtained on CSF, Blood, and other fluids
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TREATMENT
Listeriosis is a serious disease requiring
hospitalization.
A combination of antibiotics is given intravenously through a small straw-like catheter. When infection occurs during pregnancy, antibiotics must be given promptly to the mother to prevent infection of the fetus or newborn. The duration of antibiotic treatment is at least 2 weeks.
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ANTIBIOTIC TREATMENT
Ampicillin
Erythromycin Intravenous Trimethoprim
Sulphmethoxazole
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FOODS TO AVOID
Hot dogs, especially straight from the package without further heating. The fluid within hot dog packages may contain more Listeria than the hot dogs. Avoid spreading fluid from packages onto other foods, cutting boards, utensils, dishes and food preparation surfaces. Wash your hands after handling hot dogs.
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AVOID LISTERIOSIS
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Programme Created by Dr.T.V.Rao MD for Medical and Health Care Workers in the Developing World
Email doctortvrao@gmail.com
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